Generation of T cells from Human and Nonhuman Primate Pluripotent Stem Cells

Akhilesh Kumar, Saritha S D'Souza, Gene Uenishi, Mi Ae Park, Jeong Hee Lee, Igor I Slukvin

Research output: Contribution to journalArticlepeer-review

Abstract

Pluripotent stem cells (PSCs) have the potential to provide homogeneous cell populations of T cells that can be grown at a clinical scale and genetically engineered to meet specific clinical needs. OP9-DLL4, a stromal line ectopically expressing the Notch ligand Delta-like 4 (DLL4) is used to support differentiation of PSCs to T-lymphocytes. This article outlines several protocols related to generation of T cells from human and non-human primate (NHP) PSCs, including initial hematopoietic differentiation of PSC on OP9 feeders or defined conditions, followed by coculture of the OP9-DLL4 cells with the PSC-derived hematopoietic progenitors (HPs), leading to efficient differentiation to T lymphocytes. In addition, we describe a protocol for robust T cell generation from hPSCs conditionally expressing ETS1. The presented protocols provide a platform for T cell production for disease modeling and evaluating their use for immunotherapy in large animal models.

Original languageEnglish (US)
Pages (from-to)e3675
JournalBio-protocol
Volume10
Issue number13
DOIs
StatePublished - Jul 5 2020
Externally publishedYes

Bibliographical note

Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.

PubMed: MeSH publication types

  • Journal Article

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