Generation of restriction map of Enterococcus faecalis OG1 and investigation of growth requirements and regions encoding biosynthetic function

B. E. Murray, K. V. Singh, R. P. Ross, J. D. Heath, G. M. Dunny, G. M. Weinstock

Research output: Contribution to journalArticle

162 Scopus citations

Abstract

A defined synthetic medium was used to determine the amino acid requirements of Enterococcus faecalis OG1 and to demonstrate the absence of a requirement for exogenous purines or pyrimidines. Genomic libraries prepared from strain OG1 were transduced into Escherichia coli auxotrophic mutants, and cloned DNAs which complemented pyrC, pyrD, purF, purL, and guaAB mutations were identified. These and other cloned DNAs with known functions were localized on a restriction map of OG1 which was generated with SfiI (5 fragments), AscI (9 fragments), and NotI (15 fragments); the size of the OG1 chromosome was revised from a previous estimate of ~2,750 kb to 2,825 kb. The synthetic medium and the restriction map should be useful for studying enterococcal metabolic functions and the relationships between chromosomally encoded genes.

Original languageEnglish (US)
Pages (from-to)5216-5223
Number of pages8
JournalJournal of bacteriology
Volume175
Issue number16
DOIs
StatePublished - Jan 1 1993

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