Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse

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Abstract

This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1+/− heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing.

Original languageEnglish (US)
Article number122
JournalStem Cell Research and Therapy
Volume12
Issue number1
DOIs
StatePublished - Feb 12 2021

Bibliographical note

Funding Information:
We would like to acknowledge individuals from the University of Rochester, in Rochester NY, Dr. Amy Kiernan, Dr. Patricia White, Dr. J. Christopher Holt, and Dr. Lin Gan, who provided expert mentorship and training of A.S., which supported the development of this project. Additionally, we would like to acknowledge members of the Stem Cell Institute at the University of Minnesota for advice and support.

Funding Information:
Research reported in this publication was supported by the National Institute on Deafness and Other Communication Disorders of the National Institutes of Health under award number F31DC015153 (Predoctoral Fellowship, ARS) and the National Institute on Aging of the National Institute of Health under award number 2T32AG029796-11 (Postdoctoral Fellowship, ARS), and a private donation from Bridget Sperl and John McCormick supported the development of the Neurog1 mice and the acquisition of resources (PAS and WCL). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. −/−

Publisher Copyright:
© 2021, The Author(s).

Keywords

  • Blastocyst complementation
  • Inner ear
  • Neurogenin1
  • Regenerative medicine
  • Spiral ganglion neurons
  • Stem cells

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

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