Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse

Aleta R. Steevens; Matthew W. Griesbach; Yun You; James R. Dutton; Walter C. Low; Peter A. Santi

doi:10.1186/s13287-021-02184-1

Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 ^+/−−deficient mouse

Aleta R. Steevens, Matthew W. Griesbach, Yun You, James R. Dutton, Walter C. Low, Peter A. Santi

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1^+/− heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing.

Original language	English (US)
Article number	122
Journal	Stem Cell Research and Therapy
Volume	12
Issue number	1
DOIs	https://doi.org/10.1186/s13287-021-02184-1
State	Published - Feb 12 2021

Bibliographical note

Funding Information:
We would like to acknowledge individuals from the University of Rochester, in Rochester NY, Dr. Amy Kiernan, Dr. Patricia White, Dr. J. Christopher Holt, and Dr. Lin Gan, who provided expert mentorship and training of A.S., which supported the development of this project. Additionally, we would like to acknowledge members of the Stem Cell Institute at the University of Minnesota for advice and support.

Funding Information:
Research reported in this publication was supported by the National Institute on Deafness and Other Communication Disorders of the National Institutes of Health under award number F31DC015153 (Predoctoral Fellowship, ARS) and the National Institute on Aging of the National Institute of Health under award number 2T32AG029796-11 (Postdoctoral Fellowship, ARS), and a private donation from Bridget Sperl and John McCormick supported the development of the Neurog1 mice and the acquisition of resources (PAS and WCL). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. −/−

Publisher Copyright:
© 2021, The Author(s).

Keywords

Blastocyst complementation
Inner ear
Neurogenin1
Regenerative medicine
Spiral ganglion neurons
Stem cells

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural

Publisher link

10.1186/s13287-021-02184-1

Find It

Find It at U of M Libraries

Cite this

@article{9f598ab00e2b4d07bd7f2fa62a91eebc,

title = "Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse",

abstract = "This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1+/− heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing.",

keywords = "Blastocyst complementation, Inner ear, Neurogenin1, Regenerative medicine, Spiral ganglion neurons, Stem cells",

author = "Steevens, {Aleta R.} and Griesbach, {Matthew W.} and Yun You and Dutton, {James R.} and Low, {Walter C.} and Santi, {Peter A.}",

note = "Funding Information: We would like to acknowledge individuals from the University of Rochester, in Rochester NY, Dr. Amy Kiernan, Dr. Patricia White, Dr. J. Christopher Holt, and Dr. Lin Gan, who provided expert mentorship and training of A.S., which supported the development of this project. Additionally, we would like to acknowledge members of the Stem Cell Institute at the University of Minnesota for advice and support. Funding Information: Research reported in this publication was supported by the National Institute on Deafness and Other Communication Disorders of the National Institutes of Health under award number F31DC015153 (Predoctoral Fellowship, ARS) and the National Institute on Aging of the National Institute of Health under award number 2T32AG029796-11 (Postdoctoral Fellowship, ARS), and a private donation from Bridget Sperl and John McCormick supported the development of the Neurog1 mice and the acquisition of resources (PAS and WCL). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. −/− Publisher Copyright: {\textcopyright} 2021, The Author(s).",

year = "2021",

month = feb,

day = "12",

doi = "10.1186/s13287-021-02184-1",

language = "English (US)",

volume = "12",

journal = "Stem Cell Research and Therapy",

issn = "1757-6512",

publisher = "BioMed Central",

number = "1",

}

TY - JOUR

T1 - Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse

AU - Steevens, Aleta R.

AU - Griesbach, Matthew W.

AU - You, Yun

AU - Dutton, James R.

AU - Low, Walter C.

AU - Santi, Peter A.

N1 - Funding Information: We would like to acknowledge individuals from the University of Rochester, in Rochester NY, Dr. Amy Kiernan, Dr. Patricia White, Dr. J. Christopher Holt, and Dr. Lin Gan, who provided expert mentorship and training of A.S., which supported the development of this project. Additionally, we would like to acknowledge members of the Stem Cell Institute at the University of Minnesota for advice and support. Funding Information: Research reported in this publication was supported by the National Institute on Deafness and Other Communication Disorders of the National Institutes of Health under award number F31DC015153 (Predoctoral Fellowship, ARS) and the National Institute on Aging of the National Institute of Health under award number 2T32AG029796-11 (Postdoctoral Fellowship, ARS), and a private donation from Bridget Sperl and John McCormick supported the development of the Neurog1 mice and the acquisition of resources (PAS and WCL). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. −/− Publisher Copyright: © 2021, The Author(s).

PY - 2021/2/12

Y1 - 2021/2/12

N2 - This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1+/− heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing.

AB - This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1+/− heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing.

KW - Blastocyst complementation

KW - Inner ear

KW - Neurogenin1

KW - Regenerative medicine

KW - Spiral ganglion neurons

KW - Stem cells

UR - http://www.scopus.com/inward/record.url?scp=85100958740&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85100958740&partnerID=8YFLogxK

U2 - 10.1186/s13287-021-02184-1

DO - 10.1186/s13287-021-02184-1

M3 - Article

C2 - 33579352

AN - SCOPUS:85100958740

VL - 12

JO - Stem Cell Research and Therapy

JF - Stem Cell Research and Therapy

SN - 1757-6512

IS - 1

M1 - 122

ER -