Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1 +/−−deficient mouse

Aleta R. Steevens, Matthew W. Griesbach, Yun You, James R. Dutton, Walter C. Low, Peter A. Santi

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1+/− heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing.

Original languageEnglish (US)
Article number122
JournalStem Cell Research and Therapy
Volume12
Issue number1
DOIs
StatePublished - Feb 12 2021

Bibliographical note

Publisher Copyright:
© 2021, The Author(s).

Keywords

  • Blastocyst complementation
  • Inner ear
  • Neurogenin1
  • Regenerative medicine
  • Spiral ganglion neurons
  • Stem cells

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