Generation of Drosophila Heparan Sulfate Mutant Cell Lines from Existing Fly Strains

Research output: Chapter in Book/Report/Conference proceedingChapter


Genetic studies using a model organism, Drosophila melanogaster, have been contributing to elucidating the in vivo functions of heparan sulfate proteoglycans (HSPGs). On the other hand, biochemical analysis of Drosophila glycosaminoglycans (GAGs) has been limited, mainly due to the insufficient amount of the material obtained from the animal. Recently, a novel in vitro system has been developed by establishing mutant cell lines for heparan sulfate (HS)-modifying enzyme genes. Metabolic radiolabeling of GAGs allows us to assess uncharacterized features of Drosophila GAGs and the effects of the mutations on HS structures and function. The novel in vitro system will provide us with a direct link between detailed structural information of Drosophila HS and a wealth of knowledge on biological phenotypic data obtained over the last two decades using this animal model.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages10
StatePublished - 2022

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
The authors are supported by National Institutes of Health R35 GM131688 (to H. N.).

Publisher Copyright:
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.


  • Decapentaplegic
  • Drosophila
  • GAL4/UAS
  • HS
  • Mutant cell lines

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural


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