Generality of the action of various maturation-promoting factors

Takeo Kishimoto, Ryoko Kuriyama, Hisayo Kondo, Haruo Kanatani

Research output: Contribution to journalArticlepeer-review

130 Scopus citations

Abstract

It has been known in amphibians and starfishes that a cytoplasmic factor called maturation-promoting factor (MPF), produced in maturing oocytes under the influence of the maturation-inducing hormones, can induce germinal vesicle breakdown (GVBD) and the subsequent process of meiotic maturation. The present study revealed that injection of cytoplasm of maturing starfish oocytes (starfish MPF) into immature sea cucumber oocytes brought about maturation of the recipients. Amphibian MPF obtained from mature oocytes of Xenopus laevis or Bufo bufo was found to induce maturation of starfish oocytes following injection. Cytoplasm taken from cleaving starfish blastomeres induced maturation when injected into immature starfish oocytes. The maturation-inducing activity of cytoplasm of starfish blastomeres changed along with the mitotic cell cycle during 1- to 4-cell stages so far tested and reached a peak just before cleaving. Furthermore, an extract of mammalian cultured cells, CHO or V-79, synchronized in M phase, induced GVBD in starfish oocytes following injection, whereas S phase extract had little activity. These facts suggest that MPF generally brings about nuclear membrane breakdown in both meiosis and mitosis, and that the nature of MPF is very similar among vertebrates and invertebrates.

Original languageEnglish (US)
Pages (from-to)121-126
Number of pages6
JournalExperimental Cell Research
Volume137
Issue number1
DOIs
StatePublished - Jan 1982

Bibliographical note

Funding Information:
We thank Dr C. Katagiri of Hokkaido University, Dr H. Kubota of Kvoto Universitv and Dr Y. Hamada for valuabled iscussion,D r N. Takahashi of the Medical Marine Institute, Sapporo Medical College for supplying sea cucumbers, Mr H. Honda and Miss J. Ogasawaraf or collectingt oads, and Dr GrahamY oung for reading the manuscript. This work has been supported in part by grants-in-aidf rom the Ministry of Education, Science and Culture to T. K. and H. K.,

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