Gene transfer of calcium-binding proteins into adult cardiac myocytes

Brian R Thompson, Houda Cohen, Addeli Bez Batti Angulski, Joseph M Metzger

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Heart failure is the leading cause of combined morbidity and mortality in the USA with 50% of cases being diastolic heart failure. Diastolic heart failure results from poor myocardial relaxation and inadequate filling of the left ventricular chamber caused in part by calcium-handling dysregulation. In this chapter we describe methods to investigate new approaches of novel human Ca 2+ binding protein motifs to restore normal Ca 2+ handling function to diseased myocardium. Gene transfer of parvalbumin into adult cardiac myocytes has been studied as a potential therapeutic, specifically as a strategic Ca 2+ buffer to correct cardiac mechanical dysfunction in disease. This chapter provides protocols for studying wild-type parvalbumin isoforms and parvalbumins with strategically designed EF-hand motifs in adult cardiac myocytes via acute adenoviral gene transfer. These protocols have been used extensively to optimize parvalbumin function as a potential therapeutic for failing heart muscle.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages187-205
Number of pages19
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1929
ISSN (Print)1064-3745

Fingerprint

Parvalbumins
Calcium-Binding Proteins
Cardiac Myocytes
Diastolic Heart Failure
Genes
Myocardium
EF Hand Motifs
Amino Acid Motifs
Buffers
Protein Isoforms
Heart Failure
Calcium
Morbidity
Mortality
Therapeutics

Keywords

  • Adult cardiac myocyte
  • Calcium
  • Calcium imaging
  • Contractility
  • Gene transfer
  • Parvalbumin

Cite this

Thompson, B. R., Cohen, H., Angulski, A. B. B., & Metzger, J. M. (2019). Gene transfer of calcium-binding proteins into adult cardiac myocytes. In Methods in Molecular Biology (pp. 187-205). (Methods in Molecular Biology; Vol. 1929). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9030-6_12

Gene transfer of calcium-binding proteins into adult cardiac myocytes. / Thompson, Brian R; Cohen, Houda; Angulski, Addeli Bez Batti; Metzger, Joseph M.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 187-205 (Methods in Molecular Biology; Vol. 1929).

Research output: Chapter in Book/Report/Conference proceedingChapter

Thompson, BR, Cohen, H, Angulski, ABB & Metzger, JM 2019, Gene transfer of calcium-binding proteins into adult cardiac myocytes. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1929, Humana Press Inc., pp. 187-205. https://doi.org/10.1007/978-1-4939-9030-6_12
Thompson BR, Cohen H, Angulski ABB, Metzger JM. Gene transfer of calcium-binding proteins into adult cardiac myocytes. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 187-205. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9030-6_12
Thompson, Brian R ; Cohen, Houda ; Angulski, Addeli Bez Batti ; Metzger, Joseph M. / Gene transfer of calcium-binding proteins into adult cardiac myocytes. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 187-205 (Methods in Molecular Biology).
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