Gene repair and transposon-mediated gene therapy

Paul D. Richardson, Lance B. Augustin, Betsy T. Kren, Clifford J. Steer

Research output: Contribution to journalShort surveypeer-review

50 Scopus citations

Abstract

The main strategy of gene therapy has traditionally been focused on gene augmentation. This approach typically involves the introduction of an expression system designed to express a specific protein in the transfected cell. Both the basic and clinical sciences have generated enough information to suggest that gene therapy would eventually alter the fundamental practice of modern medicine. However, despite progress in the field, widespread clinical applications and success have not been achieved. The myriad deficiencies associated with gene augmentation have resulted in the development of alternative approaches to treat inherited and acquired genetic disorders. One, derived primarily from the pioneering work of homologous recombination, is gene repair. Simply stated, the process involves targeting the mutation in situ for gene correction and a return to normal gene function. Site-specific genetic repair has many advantages over augmentation although it too is associated with significant limitations. This review outlines the advantages and disadvantages of gene correction. In particular, we discuss technologies based on chimeric RNA/DNA oligonucleotides, single-stranded and triplex-forming oligonucleotides, and small fragment homologous replacement. While each of these approaches is different, they all share a number of common characteristics, including the need for efficient delivery of nucleic acids to the nucleus. In addition, we review the potential application of a novel and exciting nonviral gene augmentation strategy - the Sleeping Beauty transposon system.

Original languageEnglish (US)
Pages (from-to)105-118
Number of pages14
JournalSTEM CELLS
Volume20
Issue number2
DOIs
StatePublished - 2002

Keywords

  • Chimeraplasty
  • Chimeric RNA/DNA oligonucleotide
  • Gene repair
  • Single-stranded oligonucleotide
  • Sleeping Beauty transposon system
  • Small fragment homologous replacement
  • Triplex-forming oligonucleotide

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