Gene expression of the insulin-like growth factors and their receptors in human neuroblastoma cell lines

D. M. Martin; D. Yee; R. O. Carlson; E. L. Feldman

doi:10.1016/0169-328X(92)90114-Q

Gene expression of the insulin-like growth factors and their receptors in human neuroblastoma cell lines

D. M. Martin
, D. Yee
, R. O. Carlson
, E. L. Feldman

Medicine - Hematology, Oncology, Transplant

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Insulin-like growth factors (IGF) I and II are polypeptides with both growth-promoting and insulin-like metabolic effects. The developmentally specific expression of IGF I and II in the nervous system implies a role for these growth factors in neuronal growth and differentiation. In the present study, we analyzed IGF and IGF receptor mRNA transcripts from two related human neuroblastoma cell lines, SH-SY5Y and SK-N-SH. These cell lines provide a good in vitro model of neuronal development. Northern analysis of total RNA from each cell line revealed three IGF II mRNA transcripts (6.0, 4.8, and 1.8 kb), and one mRNA transcript each for the type I (11.0 kb) and type II (9.4 kb) IGF receptors. The size distribution of these multiple transcripts is similar to that found during normal human fetal development. These results establish both cell lines as good in vitro models for investigating the mechanisms which underly IGF gene expression during nervous system development.

Original language	English (US)
Pages (from-to)	241-246
Number of pages	6
Journal	Molecular Brain Research
Volume	15
Issue number	3-4
DOIs	https://doi.org/10.1016/0169-328X(92)90114-Q
State	Published - Oct 1992

Bibliographical note

Funding Information:
and Dr. WilliamS . Slyf or the type II IGF receptocr DNA. Funding for D.M.M. is from NIH TrainingG rantNS 07222-09D. .Y. is a Pew Scholari n the BiomedicaSl ciencesa nd is supportedb y Grant R29 CA52592. R.O.C. is supportedb y NIMH Biological SciencesR e-searchT rainingG rantM H-15794E. .L.F. is supportebdy NIH Grant NS01380.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Insulin-like growth factor I
Insulin-like growth factor II
Neuroblastoma
Type I IGF receptor
Type II IGF receptor
mRNA

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10.1016/0169-328X(92)90114-Q

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title = "Gene expression of the insulin-like growth factors and their receptors in human neuroblastoma cell lines",

abstract = "Insulin-like growth factors (IGF) I and II are polypeptides with both growth-promoting and insulin-like metabolic effects. The developmentally specific expression of IGF I and II in the nervous system implies a role for these growth factors in neuronal growth and differentiation. In the present study, we analyzed IGF and IGF receptor mRNA transcripts from two related human neuroblastoma cell lines, SH-SY5Y and SK-N-SH. These cell lines provide a good in vitro model of neuronal development. Northern analysis of total RNA from each cell line revealed three IGF II mRNA transcripts (6.0, 4.8, and 1.8 kb), and one mRNA transcript each for the type I (11.0 kb) and type II (9.4 kb) IGF receptors. The size distribution of these multiple transcripts is similar to that found during normal human fetal development. These results establish both cell lines as good in vitro models for investigating the mechanisms which underly IGF gene expression during nervous system development.",

keywords = "Insulin-like growth factor I, Insulin-like growth factor II, Neuroblastoma, Type I IGF receptor, Type II IGF receptor, mRNA",

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note = "Funding Information: and Dr. WilliamS . Slyf or the type II IGF receptocr DNA. Funding for D.M.M. is from NIH TrainingG rantNS 07222-09D. .Y. is a Pew Scholari n the BiomedicaSl ciencesa nd is supportedb y Grant R29 CA52592. R.O.C. is supportedb y NIMH Biological SciencesR e-searchT rainingG rantM H-15794E. .L.F. is supportebdy NIH Grant NS01380.",

year = "1992",

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doi = "10.1016/0169-328X(92)90114-Q",

language = "English (US)",

volume = "15",

pages = "241--246",

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AU - Martin, D. M.

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AU - Carlson, R. O.

AU - Feldman, E. L.

N1 - Funding Information: and Dr. WilliamS . Slyf or the type II IGF receptocr DNA. Funding for D.M.M. is from NIH TrainingG rantNS 07222-09D. .Y. is a Pew Scholari n the BiomedicaSl ciencesa nd is supportedb y Grant R29 CA52592. R.O.C. is supportedb y NIMH Biological SciencesR e-searchT rainingG rantM H-15794E. .L.F. is supportebdy NIH Grant NS01380.

PY - 1992/10

Y1 - 1992/10

N2 - Insulin-like growth factors (IGF) I and II are polypeptides with both growth-promoting and insulin-like metabolic effects. The developmentally specific expression of IGF I and II in the nervous system implies a role for these growth factors in neuronal growth and differentiation. In the present study, we analyzed IGF and IGF receptor mRNA transcripts from two related human neuroblastoma cell lines, SH-SY5Y and SK-N-SH. These cell lines provide a good in vitro model of neuronal development. Northern analysis of total RNA from each cell line revealed three IGF II mRNA transcripts (6.0, 4.8, and 1.8 kb), and one mRNA transcript each for the type I (11.0 kb) and type II (9.4 kb) IGF receptors. The size distribution of these multiple transcripts is similar to that found during normal human fetal development. These results establish both cell lines as good in vitro models for investigating the mechanisms which underly IGF gene expression during nervous system development.

AB - Insulin-like growth factors (IGF) I and II are polypeptides with both growth-promoting and insulin-like metabolic effects. The developmentally specific expression of IGF I and II in the nervous system implies a role for these growth factors in neuronal growth and differentiation. In the present study, we analyzed IGF and IGF receptor mRNA transcripts from two related human neuroblastoma cell lines, SH-SY5Y and SK-N-SH. These cell lines provide a good in vitro model of neuronal development. Northern analysis of total RNA from each cell line revealed three IGF II mRNA transcripts (6.0, 4.8, and 1.8 kb), and one mRNA transcript each for the type I (11.0 kb) and type II (9.4 kb) IGF receptors. The size distribution of these multiple transcripts is similar to that found during normal human fetal development. These results establish both cell lines as good in vitro models for investigating the mechanisms which underly IGF gene expression during nervous system development.

KW - Insulin-like growth factor I

KW - Insulin-like growth factor II

KW - Neuroblastoma

KW - Type I IGF receptor

KW - Type II IGF receptor

KW - mRNA

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Gene expression of the insulin-like growth factors and their receptors in human neuroblastoma cell lines

Abstract

Bibliographical note

UN SDGs

Keywords

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