Gene expression of the insulin-like growth factors and their receptors in cultured human retinal pigment epithelial cells

Donna M. Martin, Douglas Yee, Eva L. Feldman

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Insulin-like growth factors I and II (IGF I and II) are polypeptides with both growth-promoting and insulin-like metabolic effects16,27. Immunoreactive IGF I is present in the retina14 and both IGF I and II are present in vitreal fluid12. The type I and type II IGF receptors are also localized within the neural retina3,15,20,33. The presence of IGFs and IGF receptors within the eye suggests a possible growth-promoting effect of IGFs on ocular tissues. IGF may enter the eye from the blood or, alternatively, arise from an ocular cell type which synthesizes and secretes IGF. IGF I and II mRNA synthesis in scleral cells13 and IGF I synthesis in rat retina14 suggests endogenous IGF production in the eye. We hypothesized that IGFs and IGF receptors are synthesized by one ocular cell type, the retinal pigment-epithelium (RPE). As a first step in studying IGF production by the RPE, we analyzed expression of the IGF and IGF receptor genes by cultured human RPE cells. Using Northern analysis, RNase protection and reverse-transcriptase polymerase chain reaction (RT-PCR), we found that cultured RPE cells synthesize mRNA for IGF I and the type II IGF receptors.

Original languageEnglish (US)
Pages (from-to)181-186
Number of pages6
JournalMolecular Brain Research
Volume12
Issue number1-3
DOIs
StatePublished - Jan 1992

Keywords

  • Gene expression
  • Insulin-like growth factor I
  • Insulin-like growth factor II
  • Retinal pigment epithelium
  • Type I IGF receptor
  • Type II IGF receptor
  • mRNA

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