Gene expression of the insulin-like growth factors and their receptors in human neuroblastoma cell lines

D. M. Martin, D. Yee, R. O. Carlson, E. L. Feldman

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39 Scopus citations


Insulin-like growth factors (IGF) I and II are polypeptides with both growth-promoting and insulin-like metabolic effects. The developmentally specific expression of IGF I and II in the nervous system implies a role for these growth factors in neuronal growth and differentiation. In the present study, we analyzed IGF and IGF receptor mRNA transcripts from two related human neuroblastoma cell lines, SH-SY5Y and SK-N-SH. These cell lines provide a good in vitro model of neuronal development. Northern analysis of total RNA from each cell line revealed three IGF II mRNA transcripts (6.0, 4.8, and 1.8 kb), and one mRNA transcript each for the type I (11.0 kb) and type II (9.4 kb) IGF receptors. The size distribution of these multiple transcripts is similar to that found during normal human fetal development. These results establish both cell lines as good in vitro models for investigating the mechanisms which underly IGF gene expression during nervous system development.

Original languageEnglish (US)
Pages (from-to)241-246
Number of pages6
JournalMolecular Brain Research
Issue number3-4
StatePublished - Oct 1992

Bibliographical note

Funding Information:
and Dr. WilliamS . Slyf or the type II IGF receptocr DNA. Funding for D.M.M. is from NIH TrainingG rantNS 07222-09D. .Y. is a Pew Scholari n the BiomedicaSl ciencesa nd is supportedb y Grant R29 CA52592. R.O.C. is supportedb y NIMH Biological SciencesR e-searchT rainingG rantM H-15794E. .L.F. is supportebdy NIH Grant NS01380.


  • Insulin-like growth factor I
  • Insulin-like growth factor II
  • Neuroblastoma
  • Type I IGF receptor
  • Type II IGF receptor
  • mRNA


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