Gene expression atlas for the food security crop cassava

Mark C. Wilson, Andrew M. Mutka, Aaron W. Hummel, Jeffrey Berry, Raj Deepika Chauhan, Anupama Vijayaraghavan, Nigel J. Taylor, Daniel F Voytas, Daniel H. Chitwood, Rebecca S. Bart

Research output: Contribution to journalArticlepeer-review

79 Scopus citations

Abstract

Cassava (Manihot esculenta) feeds c. 800 million people world-wide. Although this crop displays high productivity under drought and poor soil conditions, it is susceptible to disease, postharvest deterioration and the roots contain low nutritional content. Here, we provide molecular identities for 11 cassava tissue/organ types through RNA-sequencing and develop an open access, web-based interface for further interrogation of the data. Through this dataset, we consider the physiology of cassava. Specifically, we focus on identification of the transcriptional signatures that define the massive, underground storage roots used as a food source and the favored target tissue for transgene integration and genome editing, friable embryogenic callus (FEC). Further, we identify promoters able to drive strong expression in multiple tissue/organs. The information gained from this study is of value for both conventional and biotechnological improvement programs.

Original languageEnglish (US)
Pages (from-to)1632-1641
Number of pages10
JournalNew Phytologist
Volume213
Issue number4
DOIs
StatePublished - Mar 1 2017

Bibliographical note

Publisher Copyright:
© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust

Keywords

  • RNA sequencing
  • biotechnology
  • cassava (Manihot esculenta)
  • food security
  • friable embryogenic callus
  • gene expression
  • organized embryogenic structures

Fingerprint

Dive into the research topics of 'Gene expression atlas for the food security crop cassava'. Together they form a unique fingerprint.

Cite this