Mesenchymal stem cells (MSCs) have been widely studied with regard to their potential use in cell therapy protocols and regenerative medicine. However, a better comprehension about the factors and molecular mechanisms driving cell differentiation is now mandatory to improve our chance to manipulate MSC behavior and to benefit future applications. In this work, we aimed to study gene regulatory networks at an early step of osteogenic differentiation. Therefore, we analyzed both the total mRNA and the mRNA fraction associated with polysomes on human adipose tissue-derived stem cells (hASCs) at 24 h of osteogenesis induction. The RNA-seq results evidenced that hASC fate is not compromised with osteogenesis at this time and that 21 days of continuous cell culture stimuli are necessary for full osteogenic differentiation of hASCs. Furthermore, early stages of osteogenesis induction involved gene regulation that was linked to the management of cell behavior in culture, such as the control of cell adhesion and proliferation. In conclusion, although discrete initial gene regulation related to osteogenesis occur, the first 24 h of induction is not sufficient to trigger and drive in vitro osteogenic differentiation of hASCs.
Bibliographical noteFunding Information:
We would like to thank all the staff of the Carlos Chagas Institute (Fiocruz-PR) for the laboratory and administrative support; the staff of the Bioinformatics Unit (Institut Pasteur Montevideo) for RNA-seq data analysis; and the staff of Cirurgia Plástica Dr. Paulo Bettes for tissue collection. We thank the Program for Technological Development in Tools for Health-PDTIS-FIOCRUZ for use of its facilities. This study was supported by Fundação Araucária and CNPq.
© 2018 The Author(s).