Gastrointestinal Microbiota Disruption and Risk of Colonization with Carbapenem-resistant Pseudomonas aeruginosa in Intensive Care Unit Patients

Melinda M. Pettigrew, Janneane F. Gent, Yong Kong, Alison Laufer Halpin, Lisa Pineles, Anthony D. Harris, J. Kristie Johnson

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Background: Carbapenem-resistant Pseudomonas aeruginosa (CRPA) colonizes the gastrointestinal tract of intensive care unit (ICU) patients, and CRPA colonization puts patients at increased risk of CRPA infection. Prior studies have not examined relationships between the microbiota, medications, and CRPA colonization acquisition. Methods: Data and perirectal swabs were obtained from a cohort of ICU patients at the University of Maryland Medical Center. Patients (N = 109) were classified into 3 groups by CRPA colonization-acquisition status and antimicrobial exposure. We conducted 16S ribosomal RNA gene sequencing of an ICU admission swab and ≥1 additional swab and evaluated associations between patient characteristics, medications, the gastrointestinal microbiota, and CRPA colonization acquisition. Results: ICU patients had low levels of diversity and high relative abundances of pathobionts. Piperacillin-tazobactam was prescribed more frequently to patients with CRPA colonization acquisition than those without. Piperacillin-tazobactam was associated with low abundance of potentially protective taxa (eg, Lactobacillus and Clostridiales) and increased risk of Enterococcus domination (odds ratio [OR], 5.50; 95% confidence interval [CI], 2.03-14.92). Opioids were associated with dysbiosis in patients who did not receive antibiotics; potentially protective Blautia and Lactobacillus were higher in patients who did not receive opioids. Several correlated taxa, identified at ICU admission, were associated with lower risk of CRPA colonization acquisition (OR, 0.58; 95% CI,. 38-.87). Conclusions: Antibiotics differed in their impact on the microbiota, with piperacillin-tazobactam being particularly damaging. Certain bacterial taxa (eg, Clostridiales) were negatively associated with CRPA colonization acquisition. These taxa may be markers of risk for CRPA colonization acquisition and/or serve a protective role.

Original languageEnglish (US)
Pages (from-to)604-613
Number of pages10
JournalClinical Infectious Diseases
Volume69
Issue number4
DOIs
StatePublished - Aug 15 2019
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2018 The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved.

Keywords

  • antibiotic resistance
  • antimicrobial stewardship
  • carbapenem-resistant Pseudomonas
  • hospital-acquired infection
  • microbiota

Fingerprint

Dive into the research topics of 'Gastrointestinal Microbiota Disruption and Risk of Colonization with Carbapenem-resistant Pseudomonas aeruginosa in Intensive Care Unit Patients'. Together they form a unique fingerprint.

Cite this