Galactofuranose side chains in galactomannans from Penicillium spp. modulate galectin-8-mediated bioactivity

Siying Zhang, Zhiying Lin, Dongmei Wang, Xuejiao Xu, Chengcheng Song, Lin Sun, Kevin H. Mayo, Zihan Zhao, Yifa Zhou

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Polysaccharides from fungi have many bioactivities. Previous studies showed that galactomannans from Penicillium oxalicum antagonize galectin-8-mediated activity. Here, two intracellular and two extracellular galactomannans were purified and their structures were comparatively characterized by NMR, partial acid hydrolysis and methylation. All four of them were identified to be galactomannans with similar mannan backbones having 1,2-/1,6-linkages (~3:1) and various amounts of galactofuranan side chains. The interaction of those polysaccharides with galectin-8 was assessed by hemagglutination and biolayer interferometry. These results show that side chains are important for the interaction, and the more the side chains, the stronger the interaction. But the side chains alone did not show act on galectin-8, which indicated that the cooperation between backbone and side chains is another necessary factor for this interaction. Our findings provide important information about structure-activity relationships and the galactofuranose-containing galactomannans might be as potential therapeutic of galectin-8 related diseases.

Original languageEnglish (US)
Article number119677
JournalCarbohydrate Polymers
StatePublished - Sep 15 2022

Bibliographical note

Funding Information:
This work was supported by the Fundamental Research Funds for the Central Universities (No:JGPY202107) and the Scientific and Technologic Foundation of Jilin Province (No:20200301018RQ).

Publisher Copyright:
© 2022


  • Galactomannan
  • Galectin-8
  • Penicillium
  • Polysaccharide
  • Structure-activity relationship

PubMed: MeSH publication types

  • Journal Article


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