Gain-of-Function Properties of a Dynamin 2 Mutant Implicated in Charcot-Marie-Tooth Disease

Tara C. Tassin, Barbara Barylko, Per Niklas Hedde, Yan Chen, Derk D. Binns, Nicholas G. James, Joachim D. Mueller, David M. Jameson, Ronald Taussig, Joseph P. Albanesi

Research output: Contribution to journalArticlepeer-review

Abstract

Mutations in the gene encoding dynamin 2 (DNM2), a GTPase that catalyzes membrane constriction and fission, are associated with two autosomal-dominant motor disorders, Charcot-Marie-Tooth disease (CMT) and centronuclear myopathy (CNM), which affect nerve and muscle, respectively. Many of these mutations affect the pleckstrin homology domain of DNM2, yet there is almost no overlap between the sets of mutations that cause CMT or CNM. A subset of CMT-linked mutations inhibit the interaction of DNM2 with phosphatidylinositol (4,5) bisphosphate, which is essential for DNM2 function in endocytosis. In contrast, CNM-linked mutations inhibit intramolecular interactions that normally suppress dynamin self-assembly and GTPase activation. Hence, CNM-linked DNM2 mutants form abnormally stable polymers and express enhanced assembly-dependent GTPase activation. These distinct effects of CMT and CNM mutations are consistent with current findings that DNM2-dependent CMT and CNM are loss-of-function and gain-of-function diseases, respectively. In this study, we present evidence that at least one CMT-causing DNM2 mutant (ΔDEE; lacking residues 555DEE557) forms polymers that, like the CNM mutants, are resistant to disassembly and display enhanced GTPase activation. We further show that the ΔDEE mutant undergoes 2-3-fold higher levels of tyrosine phosphorylation than wild-type DNM2. These results suggest that molecular mechanisms underlying the absence of pathogenic overlap between DNM2-dependent CMT and CNM should be re-examined.

Original languageEnglish (US)
Article number745940
JournalFrontiers in Cellular Neuroscience
Volume15
DOIs
StatePublished - Oct 20 2021

Bibliographical note

Funding Information:
We thank Maggy Fina (UTSW) for help in generation of constructs for Sf9 expression. The RICS and FLIM experiments reported in this publication were performed at the Laboratory for Fluorescence Dynamics (LFD) at the University of California, Irvine (UCI). The LFD is supported jointly by the National Institute of General Medical Sciences of the National Institutes of Health (P41GM103540) and UCI.

Funding Information:
This work was funded by NIH grants MH119516 (DJ and JA) and GM064589 (JM).

Publisher Copyright:
Copyright © 2021 Tassin, Barylko, Hedde, Chen, Binns, James, Mueller, Jameson, Taussig and Albanesi.

Keywords

  • centronuclear myopathy
  • Charcot-Marie-Tooth disease
  • dynamin
  • FLIM/FRET
  • fluorescence fluctuation spectroscopy
  • tyrosine phosphorylation

PubMed: MeSH publication types

  • Journal Article

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