Gag p24 Is a Marker of Human Immunodeficiency Virus Expression in Tissues and Correlates with Immune Response

Guoxin Wu; Paul Zuck; Shih Lin Goh; Jeffrey M. Milush; Poonam Vohra; Joseph K. Wong; Ma Somsouk; Steven A. Yukl; Barbara L. Shacklett; Nicolas Chomont; Ashley T. Haase; Hiroyu Hatano; Timothy W. Schacker; Steven G. Deeks; Daria J. Hazuda; Peter W. Hunt; Bonnie J. Howell

doi:10.1093/infdis/jiab121

Gag p24 Is a Marker of Human Immunodeficiency Virus Expression in Tissues and Correlates with Immune Response

Guoxin Wu, Paul Zuck, Shih Lin Goh, Jeffrey M. Milush, Poonam Vohra, Joseph K. Wong, Ma Somsouk, Steven A. Yukl, Barbara L. Shacklett, Nicolas Chomont, Ashley T. Haase, Hiroyu Hatano, Timothy W. Schacker, Steven G. Deeks, Daria J. Hazuda, Peter W. Hunt, Bonnie J. Howell

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15 Scopus citations

Abstract

We demonstrate that human immunodeficiency virus (HIV) gag p24 protein is more readily detected in gut and lymph node tissues than in blood CD4+ T cells and correlates better with CD4 count during antiretroviral therapy (ART). Gut p24 levels also measurably decline with ART in natural controllers. During ART, gut p24 expression is more strongly associated both with HIV-specific CD8+ T-cell frequency and plasma soluble CD14 levels than gut HIV RNA expression. This study supports using gag p24 as a marker of HIV expression in HIV+ tissues to study effects of viral persistence and to monitor efficacy of treatment in HIV-based clearance studies.

Original language	English (US)
Pages (from-to)	1593-1598
Number of pages	6
Journal	Journal of Infectious Diseases
Volume	224
Issue number	9
DOIs	https://doi.org/10.1093/infdis/jiab121
State	Published - Nov 1 2021

Bibliographical note

Funding Information:
Previously reported data [1] and additional work referenced in this manuscript were supported by grants from the National Institute of Allergy and Infectious Diseases (R01 AI087145, K23 AI075985, K24 AI069994, R56 AI091573, R01 NS051132, R01 AI057020); National Cancer Institute (K23 CA157929); Delaney AIDS Research Enterprise (DARE) to Find a Cure (1U19AI096109, 1UM1AI126611?01); California HIV/AIDS Research Program (ID08-SF-004); American Foundation for AIDS Research (106710-40-RGRL, 107170-44-RGRL, 108073-50-RGRL); University of California, San Francisco (UCSF)/Gladstone Institute of Virology and Immunology Center for AIDS Research (CFAR) (P30 AI027763); UCSF Clinical and Translational Research Institute Clinical Research Center (UL1 RR024131); Center for AIDS Prevention Studies (P30 MH62246); CFAR Network of Integrated Systems (R24 AI067039); and US Department of Veterans Affairs (1 IK2 CX000520-01, 5101BX001048).

Publisher Copyright:
© 2021 The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].

Keywords

HIV
HIV persistence
biomarker
fine needle aspirates
gag p24
lymph nodes
rectal biopsy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/infdis/jiab121

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Wu, G., Zuck, P., Goh, S. L., Milush, J. M., Vohra, P., Wong, J. K., Somsouk, M., Yukl, S. A., Shacklett, B. L., Chomont, N., Haase, A. T., Hatano, H., Schacker, T. W., Deeks, S. G., Hazuda, D. J., Hunt, P. W., & Howell, B. J. (2021). Gag p24 Is a Marker of Human Immunodeficiency Virus Expression in Tissues and Correlates with Immune Response. Journal of Infectious Diseases, 224(9), 1593-1598. https://doi.org/10.1093/infdis/jiab121

Wu, G, Zuck, P, Goh, SL, Milush, JM, Vohra, P, Wong, JK, Somsouk, M, Yukl, SA, Shacklett, BL, Chomont, N, Haase, AT, Hatano, H, Schacker, TW, Deeks, SG, Hazuda, DJ, Hunt, PW & Howell, BJ 2021, 'Gag p24 Is a Marker of Human Immunodeficiency Virus Expression in Tissues and Correlates with Immune Response', Journal of Infectious Diseases, vol. 224, no. 9, pp. 1593-1598. https://doi.org/10.1093/infdis/jiab121

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abstract = "We demonstrate that human immunodeficiency virus (HIV) gag p24 protein is more readily detected in gut and lymph node tissues than in blood CD4+ T cells and correlates better with CD4 count during antiretroviral therapy (ART). Gut p24 levels also measurably decline with ART in natural controllers. During ART, gut p24 expression is more strongly associated both with HIV-specific CD8+ T-cell frequency and plasma soluble CD14 levels than gut HIV RNA expression. This study supports using gag p24 as a marker of HIV expression in HIV+ tissues to study effects of viral persistence and to monitor efficacy of treatment in HIV-based clearance studies.",

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author = "Guoxin Wu and Paul Zuck and Goh, {Shih Lin} and Milush, {Jeffrey M.} and Poonam Vohra and Wong, {Joseph K.} and Ma Somsouk and Yukl, {Steven A.} and Shacklett, {Barbara L.} and Nicolas Chomont and Haase, {Ashley T.} and Hiroyu Hatano and Schacker, {Timothy W.} and Deeks, {Steven G.} and Hazuda, {Daria J.} and Hunt, {Peter W.} and Howell, {Bonnie J.}",

note = "Funding Information: Previously reported data [1] and additional work referenced in this manuscript were supported by grants from the National Institute of Allergy and Infectious Diseases (R01 AI087145, K23 AI075985, K24 AI069994, R56 AI091573, R01 NS051132, R01 AI057020); National Cancer Institute (K23 CA157929); Delaney AIDS Research Enterprise (DARE) to Find a Cure (1U19AI096109, 1UM1AI126611?01); California HIV/AIDS Research Program (ID08-SF-004); American Foundation for AIDS Research (106710-40-RGRL, 107170-44-RGRL, 108073-50-RGRL); University of California, San Francisco (UCSF)/Gladstone Institute of Virology and Immunology Center for AIDS Research (CFAR) (P30 AI027763); UCSF Clinical and Translational Research Institute Clinical Research Center (UL1 RR024131); Center for AIDS Prevention Studies (P30 MH62246); CFAR Network of Integrated Systems (R24 AI067039); and US Department of Veterans Affairs (1 IK2 CX000520-01, 5101BX001048). Publisher Copyright: {\textcopyright} 2021 The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].",

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AU - Zuck, Paul

AU - Goh, Shih Lin

AU - Milush, Jeffrey M.

AU - Vohra, Poonam

AU - Wong, Joseph K.

AU - Somsouk, Ma

AU - Yukl, Steven A.

AU - Shacklett, Barbara L.

AU - Chomont, Nicolas

AU - Haase, Ashley T.

AU - Hatano, Hiroyu

AU - Schacker, Timothy W.

AU - Deeks, Steven G.

AU - Hazuda, Daria J.

AU - Hunt, Peter W.

AU - Howell, Bonnie J.

N1 - Funding Information: Previously reported data [1] and additional work referenced in this manuscript were supported by grants from the National Institute of Allergy and Infectious Diseases (R01 AI087145, K23 AI075985, K24 AI069994, R56 AI091573, R01 NS051132, R01 AI057020); National Cancer Institute (K23 CA157929); Delaney AIDS Research Enterprise (DARE) to Find a Cure (1U19AI096109, 1UM1AI126611?01); California HIV/AIDS Research Program (ID08-SF-004); American Foundation for AIDS Research (106710-40-RGRL, 107170-44-RGRL, 108073-50-RGRL); University of California, San Francisco (UCSF)/Gladstone Institute of Virology and Immunology Center for AIDS Research (CFAR) (P30 AI027763); UCSF Clinical and Translational Research Institute Clinical Research Center (UL1 RR024131); Center for AIDS Prevention Studies (P30 MH62246); CFAR Network of Integrated Systems (R24 AI067039); and US Department of Veterans Affairs (1 IK2 CX000520-01, 5101BX001048). Publisher Copyright: © 2021 The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].

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N2 - We demonstrate that human immunodeficiency virus (HIV) gag p24 protein is more readily detected in gut and lymph node tissues than in blood CD4+ T cells and correlates better with CD4 count during antiretroviral therapy (ART). Gut p24 levels also measurably decline with ART in natural controllers. During ART, gut p24 expression is more strongly associated both with HIV-specific CD8+ T-cell frequency and plasma soluble CD14 levels than gut HIV RNA expression. This study supports using gag p24 as a marker of HIV expression in HIV+ tissues to study effects of viral persistence and to monitor efficacy of treatment in HIV-based clearance studies.

AB - We demonstrate that human immunodeficiency virus (HIV) gag p24 protein is more readily detected in gut and lymph node tissues than in blood CD4+ T cells and correlates better with CD4 count during antiretroviral therapy (ART). Gut p24 levels also measurably decline with ART in natural controllers. During ART, gut p24 expression is more strongly associated both with HIV-specific CD8+ T-cell frequency and plasma soluble CD14 levels than gut HIV RNA expression. This study supports using gag p24 as a marker of HIV expression in HIV+ tissues to study effects of viral persistence and to monitor efficacy of treatment in HIV-based clearance studies.

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KW - biomarker

KW - fine needle aspirates

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KW - lymph nodes

KW - rectal biopsy

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ER -

Gag p24 Is a Marker of Human Immunodeficiency Virus Expression in Tissues and Correlates with Immune Response

Abstract

Bibliographical note

Keywords

UN SDGs

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