G protein effects on Ca2+ release and excitation-contraction coupling in skeletal muscle fibers

L. Carney-Anderson, S. K. Donaldson

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Skeletal muscle excitation-contraction (EC) coupling may involve secondary mechanisms, such as those involving G proteins. The aim of this study was to identify possible G protein effects on sarcoplasmic reticulum (SR) Ca2+ release, in general, and on voltage-dependent EC coupling, in particular. Effects of guanosine 5'-O-(3-thiotriphosphate) (GTPγS) were studied using a single peeled rabbit skeletal muscle fiber preparation that is capable of releasing SR Ca2+ in response to transverse tubule (TT) depolarization. Because of possible nonspecific and residual effects of 200 μM GTPγS, a lower concentration of 50 μM GTPγS was used to stimulate G proteins in the peeled fiber system. Under conditions for steady state (resting) polarization of TT, GTPγS rarely elicited SR Ca2+ release. When the TTs are in steady- state (resting) depolarization, 50 μM GTPγS or GTP elicited SR Ca2+ release and associated tension transients in only 69% of fibers tested. In contrast, GTPγS always augmented Ca2+ release during TT depolarization- induced EC coupling. These results indicate the presence of at least two excitatory G proteins for SR Ca2+ release, only one of which is a modulator albeit nonessential, of peeled fiber EC coupling.

Original languageEnglish (US)
Pages (from-to)C1087-C1094
JournalAmerican Journal of Physiology - Cell Physiology
Volume267
Issue number4 part 1
DOIs
StatePublished - 1994

Keywords

  • caffeine
  • guanosine 5'-O-(2-thiodiphosphate)
  • guanosine 5'-O-(3- thiotriphosphate
  • peeled skeletal muscle fibers
  • rabbit
  • sarcoplasmic reticulum calcium release

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