Fungal degradation of complex organic carbon supports denitrification in saturated woodchip bioreactors

Woodchip bioreactor (WBR) is a promising technology for the removal of nitrate from agricultural drainage, although the performance of WBRs is dependent on the decomposition of lignocellulosic biomass and the carbon availability for microbial denitrification. Fungal species are more efficient than bacterial counterparts in driving wood decomposition; however, little is known about the fungal community structure and functions in saturated WBRs. In this study, we investigated the dynamics of the mycobiome in field-scale, constantly saturated WBRs located in Willmar, Minnesota, USA. Fungal community analysis suggested that wood-rotting fungi were abundant in WBRs, especially near their inlet locations where microbial denitrification was most active. Complex network structures of fungal hyphae associated with a decayed cavity on the woodchip surface was further evidenced by confocal and scanning electron microscopy. These results suggest that fungi play a major role in wood degradation in WBRs, thereby promoting denitrification activity.