Functionalization of single-walled carbon nanotubes enables efficient intracellular delivery of siRNA targeting MDM2 to inhibit breast cancer cells growth

Hailong Chen, Xingyuan Ma, Zhi Li, Qiaoyun Shi, Wenyun Zheng, Yang Liu, Ping Wang

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

The delivery of DNA or RNA to cells represents the limiting step in the development of cancer gene therapy and RNA interference protocols. Single walled carbon nanotubes (SWNTs) are of interest as carriers of biologically active molecules because of their ability to cross cell membranes. In this study, we developed a novel strategy for chemical functionalization of SWNTs (f-SWCNTs) with DSPE-PEG-Amine to bind small interfering RNA (siRNA) by disulfide bonds applied to siRNA-mediated gene silencing in breast cancer cells. Results indicated the efficiency of f-SWNTs carrying siRNA reached 83.55%, and the new f-SWNTs-siRNA-MDM2 complexes were successfully introduced into the breast carcinoma B-Cap-37 cells at a concentration of 100. nM in mediums, and caused proliferation inhibition of B-Cap-37 cells significantly. The proliferation inhibition ratio of B-Cap-37 cells was detected as 44.53% for 72. h, and the apoptosis ratio was measured as 30.45%. It was obvious that MDM2 can serve as a novel therapeutic target by an effective carrier system of DSPE-PEG-Amine-functionalized SWNTs, which would be very advanced and significant to therapy of breast cancer further.

Original languageEnglish (US)
Pages (from-to)334-338
Number of pages5
JournalBiomedicine and Pharmacotherapy
Volume66
Issue number5
DOIs
StatePublished - Jul 2012

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation (30873190), the National Science Research Project “Significant New Drugs Created” of Eleventh Five-year Plan (2009ZX09103-693), Key grant cultivated the project and cross-disciplinary of Chinese Ministry and partially supported by open funding project of the State Key Laboratory of Bioreactor Engineering.

Keywords

  • Breast cancer cells
  • Delivery of siRNA
  • Functionalized SWNTs
  • MDM2

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