Abstract
Hepatic inflammation, driven by immune cells such as B and T lymphocytes, is a hallmark feature of metabolic dysfunction-associated steatohepatitis (MASH). Here, we detail a robust cytometry by time-of-flight (CyTOF) procedure to phenotype hepatic lymphocytes from mice with MASH. We employ custom metal conjugation of antibodies, isolation of hepatic lymphocytes, cell surface and intracellular staining, and data acquisition. This protocol overcomes the limitations of traditional flow cytometry by accommodating up to 40 markers for comprehensive immune phenotyping. For complete details on the use and execution of this protocol, please refer to Barrow et al.1
Original language | English (US) |
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Article number | 102743 |
Journal | STAR Protocols |
Volume | 4 |
Issue number | 4 |
DOIs | |
State | Published - Dec 15 2023 |
Bibliographical note
Publisher Copyright:© 2023 The Authors
Keywords
- Antibody
- Cell isolation
- High Throughput Screening
- Immunology
- Mass Cytometry
- Metabolism
- Molecular/Chemical Probes
- Single Cell