Functional Myogenic Engraftment from Mouse iPS Cells

Radbod Darabi, Weihong Pan, Darko Bosnakovski, June Baik, Michael Kyba, Rita C R Perlingeiro

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99 Scopus citations


Direct reprogramming of adult fibroblasts to a pluripotent state has opened new possibilities for the generation of patient- and disease-specific stem cells. However the ability of induced pluripotent stem (iPS) cells to generate tissue that mediates functional repair has been demonstrated in very few animal models of disease to date. Here we present the proof of principle that iPS cells may be used effectively for the treatment of muscle disorders. We combine the generation of iPS cells with conditional expression of Pax7, a robust approach to derive myogenic progenitors. Transplantation of Pax7-induced iPS-derived myogenic progenitors into dystrophic mice results in extensive engraftment, which is accompanied by improved contractility of treated muscles. These findings demonstrate the myogenic regenerative potential of iPS cells and provide rationale for their future therapeutic application for muscular dystrophies.

Original languageEnglish (US)
Pages (from-to)948-957
Number of pages10
JournalStem Cell Reviews and Reports
Issue number4
StatePublished - Nov 2011

Bibliographical note

Funding Information:
Acknowledgments The project was supported by NIH grants RC1AR058118 to RCRP and R01 HL081186 to MK and by the Dr. Bob and Jean Smith Foundation. The monoclonal antibody to MHC was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa.


  • Muscular dystrophy
  • Pax7
  • Skeletal muscle progenitors
  • iPS cells


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