Functional expression of a mammalian acetylcholinesterase in Pichia pastoris: Comparison to acetylcholinesterase, expressed and reconstituted from Escherichia coli

Immunology and Microbiology

• Inclusion Bodies 100%
• Escherichia coli 100%
• Komagataella pastoris 100%
• Carboxy Terminal Sequence 66%
• Expression Vector 66%
• Affinity Chromatography 33%
• Periplasmic Space 33%
• Chromatography 33%
• Secretion (Process) 33%
• Promoter Region 33%

Keyphrases

• Escherichia Coli 100%
• Acetylcholinesterase 100%
• Pichia Pastoris 100%
• Functional Expression 100%
• Rat Brain 11%
• Expression Vector 11%
• Brain Acetylcholinesterase 11%
• Inclusion Bodies 11%
• Overexpression 5%
• Specific Activity 5%
• Physicochemical Properties 5%
• C-terminus 5%
• Cysteine Residues 5%
• Affinity Chromatography 5%
• Good Yield 5%
• Signal Peptide 5%
• Culture Conditions 5%
• Temperature-inducible 5%
• 6xHis Tag 5%
• Refolding Efficiency 5%
• Active Enzyme 5%
• Mature Rats 5%
• OmpA Protein 5%
• Periplasmic Space 5%
• Acetylcholinesterase Gene 5%
• Ni-NTA Affinity Chromatography 5%
• Inducible Promoter 5%
• Inhibition Properties 5%
• Cytoplasmic Inclusion Bodies 5%
• Microbial Host 5%

Neuroscience

• Acetylcholinesterase 100%
• C-Terminus 10%
• Expression Vector 10%
• In Vitro 5%
• Promoter Region 5%
• Periplasm 5%
• Cysteine 5%
• Outer Membrane Protein A 5%
• Signal Peptide 5%
• Affinity Chromatography 5%