Functional characterization of PCRK1, a putative protein kinase with a role in immunity

Suma Sreekanta, Miyoshi Haruta, Benjamin B. Minkoff, Jane Glazebrook

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


In Arabidopsis, defense signaling is triggered by the perception of conserved molecular patterns by pattern recognition receptors (PRRs). Signal transduction from the PRRs requires members of a family of Receptor-Like Cytoplasmic Kinases (RLCKs). Previously, we described one such RLCK, PTI Compromised Receptor-Like Cytoplasmic Kinase 1 (PCRK1) that is important for immunity induced by Microbe Associated Molecular Patterns (MAMPs) as well as Damage Associated Molecular Patterns (DAMPs). In this study, we measured the growth of Pma ES4326 in double mutants carrying pcrk1 together with the salicylic acid (SA) biosynthesis mutation sid2-2 or the jasmonic acid (JA) receptor mutation coi1-1, showing that the function of PCRK1 is SA independent but may be partially dependent on JA. Mutation of phosphorylated serine residues S232, S233 and S237 compromised the immune signaling function of PCRK1.

Original languageEnglish (US)
Article numbere1063759
JournalPlant Signaling and Behavior
Issue number10
StatePublished - 2015

Bibliographical note

Funding Information:
This work was supported by grants IOS-0925375 and MCB-0929395 from the National Science Foundation to J.G. and Michael R. Sussman, respectively. B.B.M. was supported by NHGRI training grant 5T32HG002760 to the Genomic Science Training Program.

Publisher Copyright:
© 2015 Taylor & Francis Group, LLC.


  • Microbe-associated molecular pattern
  • Pattern Triggered Immunity (PTI)
  • Receptor-like cytoplasmic kinase


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