Functional aspects of oxidative phosphorylation and electron transport in cardiac mitochondria of copper-deficient rats

J. M. Matz, J. T. Saari, A. M. Bode

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Although dietary copper deficiency causes physiological, morphological, and biochemical abnormalities in cardiac mitochondria, the relationship observed between abnormalities of mitochondrial structure and function have been inconsistent in previous studies. The purpose of the present study was to re-evaluate the respiration rates of cardiac mitochondria from copper-deficient rats and to use several drugs that uncouple and inhibit mitochondrial respiration in order to clarify the mechanisms of mitochondrial dysfunction found in several laboratories. Copper deficiency reduced state 4 and state 3 cardiac mitochondrial respiration rates with all substrates tested. However, neither the ratio of ADP/oxygen consumed nor the acceptor control index was affected by copper deficiency. Cardiac mitochondria of copper-deficient rats showed a resistance to respiratory blockade by oligomycin and an increased ability to hydrolyze ATP in the presence of oligomycin compared with mitochondria of copper-adequate rats. This suggests that copper deficiency affects the function of the cardiac mitochondrial ATP synthase.

Original languageEnglish (US)
Pages (from-to)644-652
Number of pages9
JournalThe Journal of Nutritional Biochemistry
Volume6
Issue number12
DOIs
StatePublished - Dec 1995
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported in part by the USDA, ARS. Mention of a trademark or propriety product does not constitute a guarantee or warranty of the product by the US Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable. This is a US government work. There are no restrictions on its use. The U.S. Department of Agriculture, Agricultural Research Service, Northern Plains Area, is an equal opportunity/affirmative action employer and all agency services are available without discrimination. We thank Jackie Keith and Gwen Schelkoph for their technical service and assistance .

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

Keywords

  • ATP synthase
  • electron transport
  • mitochondrial respiration
  • oxidative phosphorylation

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