Full-thickness human skin equivalent models of atopic dermatitis

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Atopic dermatitis is a chronic inflammatory skin disease caused by complex multifactorial etiology. In the recent years, there have been significant advances in tissue engineering and the generation of in vitro skin models representative of healthy and diseased states. This chapter describes the methodology for the fabrication of in vitro human skin equivalent (HSE) from human keratinocytes and fibroblasts using a fibrin-based dermal matrix and serum-free culture conditions. Modification of the culture conditions with the supplementation of Th2 cytokines such as interleukin-4 induces the development of atopic dermatitis-like skin model. The chapter also describes the histological and immunohistochemical tools for characterization of the HSE model. The reconstruction of tissue-engineered HSE models that recapitulate the essential features of atopic dermatitis provides powerful tools for deeper understanding of the underlying pathological mechanisms on epidermal level, identification and testing of novel treatment options, and safety and toxicological evaluation in a pathophysiologically relevant system.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages367-383
Number of pages17
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1879
ISSN (Print)1064-3745

Fingerprint

Atopic Dermatitis
Skin
Tissue Engineering
Fibrin
Keratinocytes
Skin Diseases
Interleukin-4
Toxicology
Fibroblasts
Cytokines
Safety
Serum

Keywords

  • 3D tissue constructs
  • Atopic dermatitis
  • Fibrin
  • Full-thickness
  • Human skin equivalent
  • Organoids
  • Organotypic culture
  • Tissue engineering

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Sriram, G., Bigliardi, P. L., & Bigliardi-Qi, M. (2019). Full-thickness human skin equivalent models of atopic dermatitis. In Methods in Molecular Biology (pp. 367-383). (Methods in Molecular Biology; Vol. 1879). Humana Press Inc.. https://doi.org/10.1007/7651_2018_163

Full-thickness human skin equivalent models of atopic dermatitis. / Sriram, Gopu; Bigliardi, Paul L; Bigliardi-Qi, Mei.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 367-383 (Methods in Molecular Biology; Vol. 1879).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sriram, G, Bigliardi, PL & Bigliardi-Qi, M 2019, Full-thickness human skin equivalent models of atopic dermatitis. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1879, Humana Press Inc., pp. 367-383. https://doi.org/10.1007/7651_2018_163
Sriram G, Bigliardi PL, Bigliardi-Qi M. Full-thickness human skin equivalent models of atopic dermatitis. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 367-383. (Methods in Molecular Biology). https://doi.org/10.1007/7651_2018_163
Sriram, Gopu ; Bigliardi, Paul L ; Bigliardi-Qi, Mei. / Full-thickness human skin equivalent models of atopic dermatitis. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 367-383 (Methods in Molecular Biology).
@inbook{c48266379a8147f3b0138178fa758bac,
title = "Full-thickness human skin equivalent models of atopic dermatitis",
abstract = "Atopic dermatitis is a chronic inflammatory skin disease caused by complex multifactorial etiology. In the recent years, there have been significant advances in tissue engineering and the generation of in vitro skin models representative of healthy and diseased states. This chapter describes the methodology for the fabrication of in vitro human skin equivalent (HSE) from human keratinocytes and fibroblasts using a fibrin-based dermal matrix and serum-free culture conditions. Modification of the culture conditions with the supplementation of Th2 cytokines such as interleukin-4 induces the development of atopic dermatitis-like skin model. The chapter also describes the histological and immunohistochemical tools for characterization of the HSE model. The reconstruction of tissue-engineered HSE models that recapitulate the essential features of atopic dermatitis provides powerful tools for deeper understanding of the underlying pathological mechanisms on epidermal level, identification and testing of novel treatment options, and safety and toxicological evaluation in a pathophysiologically relevant system.",
keywords = "3D tissue constructs, Atopic dermatitis, Fibrin, Full-thickness, Human skin equivalent, Organoids, Organotypic culture, Tissue engineering",
author = "Gopu Sriram and Bigliardi, {Paul L} and Mei Bigliardi-Qi",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/7651_2018_163",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "367--383",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Full-thickness human skin equivalent models of atopic dermatitis

AU - Sriram, Gopu

AU - Bigliardi, Paul L

AU - Bigliardi-Qi, Mei

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Atopic dermatitis is a chronic inflammatory skin disease caused by complex multifactorial etiology. In the recent years, there have been significant advances in tissue engineering and the generation of in vitro skin models representative of healthy and diseased states. This chapter describes the methodology for the fabrication of in vitro human skin equivalent (HSE) from human keratinocytes and fibroblasts using a fibrin-based dermal matrix and serum-free culture conditions. Modification of the culture conditions with the supplementation of Th2 cytokines such as interleukin-4 induces the development of atopic dermatitis-like skin model. The chapter also describes the histological and immunohistochemical tools for characterization of the HSE model. The reconstruction of tissue-engineered HSE models that recapitulate the essential features of atopic dermatitis provides powerful tools for deeper understanding of the underlying pathological mechanisms on epidermal level, identification and testing of novel treatment options, and safety and toxicological evaluation in a pathophysiologically relevant system.

AB - Atopic dermatitis is a chronic inflammatory skin disease caused by complex multifactorial etiology. In the recent years, there have been significant advances in tissue engineering and the generation of in vitro skin models representative of healthy and diseased states. This chapter describes the methodology for the fabrication of in vitro human skin equivalent (HSE) from human keratinocytes and fibroblasts using a fibrin-based dermal matrix and serum-free culture conditions. Modification of the culture conditions with the supplementation of Th2 cytokines such as interleukin-4 induces the development of atopic dermatitis-like skin model. The chapter also describes the histological and immunohistochemical tools for characterization of the HSE model. The reconstruction of tissue-engineered HSE models that recapitulate the essential features of atopic dermatitis provides powerful tools for deeper understanding of the underlying pathological mechanisms on epidermal level, identification and testing of novel treatment options, and safety and toxicological evaluation in a pathophysiologically relevant system.

KW - 3D tissue constructs

KW - Atopic dermatitis

KW - Fibrin

KW - Full-thickness

KW - Human skin equivalent

KW - Organoids

KW - Organotypic culture

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=85060629602&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060629602&partnerID=8YFLogxK

U2 - 10.1007/7651_2018_163

DO - 10.1007/7651_2018_163

M3 - Chapter

T3 - Methods in Molecular Biology

SP - 367

EP - 383

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -