F2 screen, inheritance and cross-resistance of field-derived Vip3A resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) collected from Louisiana, USA

Fei Yang, Shannon Morsello, Graham P. Head, Chris Sansone, Fangneng Huang, Ryan T. Gilreath, David L. Kerns

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

BACKGROUND: Fall armyworm, Spodoptera frugiperda, is a target pest of the Vip3A protein used in pyramided Bt corn and cotton in the USA. In this study, we provide the first documentation of a resistance allele conferring Vip3A resistance in a field-derived population of S. frugiperda from the USA, and characterize its inheritance and cross-resistance. RESULTS: An F2 screen with 104 two-parent families generated from a field collection of S. frugiperda in Louisiana, USA, resulted in one family carrying a Vip3A resistance allele. The Vip3A-resistant strain (RR) derived from the two-parent family showed a high level of resistance to Vip3A in both diet and whole-plant bioassays, with a resistance ratio of >632.0-fold relative to a susceptible population (SS) based on diet-overlay bioassays. The inheritance of Vip3A resistance was monogenic, autosomal and recessive. Furthermore, the Vip3A resistance conferred no cross-resistance to Cry1F, Cry2Ab2 or Cry2Ae purified proteins, with resistance ratios of 3.5, 5.0 and 1.1, respectively. CONCLUSION: These findings provide valuable information for characterizing Vip3A resistance, resistance monitoring, and developing effective resistance management strategies for the sustainable use of the Vip3A technology.

Original languageEnglish (US)
Pages (from-to)1769-1778
Number of pages10
JournalPest management science
Volume74
Issue number8
DOIs
StatePublished - Aug 2018
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2017 Society of Chemical Industry

Keywords

  • Bacillus thuringiensis
  • F screen
  • Spodoptera frugiperda
  • Vip3A
  • cross-resistance
  • inheritance
  • resistance

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