Frequency of resistance to Bacillus thuringiensis toxin Cry1Ab in an Iowa population of European corn borer (lepidoptera: crambidae)

D. A. Andow, D. M. Olson, R. L. Hellmich, D. N. Alstad, W. D. Hutchison

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

The refuge plus high-dose strategy for resistance management assumes that the frequency of resistance alleles is low. We used an F2 screen to estimate the frequency of resistance to transgenic corn that produces Bacillus thuringiensis Berliner Cry1Ab toxin (Bt corn) in an Iowa population of European corn borer, Ostrinia nubilalis (Hübner). We also proposed a modification to the statistical analysis of the F2 screen that extends its application for nonuniform prior distributions and for repeated sampling of a single population. Based on a sample of 188 isofemale lines derived from females caught at light traps during the 2nd flight of 1997, we show with 95% confidence that the frequency of resistance to Bt corn was <3.9 × 10-3 in this Iowa population. These results provide weak evidence that the refuge plus high-dose strategy may be effective for managing resistance in O. nubilalis to Bt corn. Partial resistance to Cry1Ab toxin was found commonly. The 95% CI for the frequency of partial resistance were [8.2 × 10-4, 9.4 × 10-3] for the Iowa population. Variable costs of the method were $14.90 per isofemale line, which was a reduction of 25% compared with our initial estimate.

Original languageEnglish (US)
Pages (from-to)26-30
Number of pages5
JournalJournal of economic entomology
Volume93
Issue number1
StatePublished - Dec 1 2000

Keywords

  • F screen
  • Ostrinia nubilalis
  • Resistance management
  • Transgenic corn

Fingerprint Dive into the research topics of 'Frequency of resistance to Bacillus thuringiensis toxin Cry1Ab in an Iowa population of European corn borer (lepidoptera: crambidae)'. Together they form a unique fingerprint.

  • Cite this