Frequency of Cry1F resistance alleles in Spodoptera frugiperda (Lepidoptera: Noctuidae) in Brazil

Juliano R. Farias, David A. Andow, Renato J. Horikoshi, Daniel Bernardi, Rebeca da S Ribeiro, Antonio Rb do Nascimento, Antonio C dos Santos, Celso Omoto

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

BACKGROUND: The frequency of resistance alleles is a major factor influencing the rate of resistance evolution. Here, we adapted the F2 screen procedure for Spodoptera frugiperda (J. E. Smith) with a discriminating concentration assay, and extended associated statistical methods to estimate the frequency of resistance to Cry1F protein in S. frugiperda in Brazil when resistance was not rare. RESULTS: We show that F2 screen is efficient even when the resistance frequency is 0.250. It was possible to screen 517 isoparental lines from 12 populations sampled in five states of Brazil during the first half of 2012. Western Bahia had the highest allele frequency of Cry1F resistance, 0.192, with a 95% confidence interval (CI) between 0.163 and 0.220. All other states had a similar and lower frequency varying from 0.042 in Paraná to 0.080 in Mato Grosso do Sul. CONCLUSION: The high frequency in western Bahia may be related to year-round availability of maize, the high population density of S. frugiperda, the lack of refuges and the high adoption rate of Cry1F maize. Cry1F resistance alleles were not rare and occurred at frequencies that have already compromised the useful life of TC1507 maize in western Bahia.

Original languageEnglish (US)
Pages (from-to)2295-2302
Number of pages8
JournalPest management science
Volume72
Issue number12
DOIs
StatePublished - Dec 1 2016

Keywords

  • Bacillus thuringiensis
  • Bt maize
  • TC1507 maize
  • fall armyworm
  • resistance management

Fingerprint Dive into the research topics of 'Frequency of Cry1F resistance alleles in Spodoptera frugiperda (Lepidoptera: Noctuidae) in Brazil'. Together they form a unique fingerprint.

Cite this