TY - JOUR
T1 - Frequency of circulating iNKT cells among Iranian healthy adults
AU - Fereidouni, Mohammad
AU - Hosseini, Reza Farid
AU - Azad, Farahzad Jabbari
AU - Schenkel, Jason
AU - Varasteh, Abdolreza
AU - Mahmoudi, Mahmoud
PY - 2010
Y1 - 2010
N2 - Background: Invariant natural killer T cells (iNKT) are a small subset of T lymphocytes which are involved in a wide variety of immune responses. Human studies have reported a wide variation in the number of circulating iNKT cell among healthy donors as well as in some immunologic disorders while there is no or little data about the factors which affect the pool of circulating iNKT cells in human. Given the importance of iNKT cells in immunologic responses and variation in the number and pheno-type of iNKT cells, the aim of this study was to evaluate the frequency of circulating iNKT cells in a cohort of healthy Iranian adults. Methods: The number of circulating iNKT cells was measured among PBMC in a group of healthy Iranian adults by flow cytometry. iNKT cells were identified by using 6B11 monoclonal antibody or by coexpression of TCR Vα24 and Vα11 chain. Results: The mean frequency of 6B11-iNKT cells and Vα24-Vβ11 iNKT cells among T lymphocytes, were 0.54% and 0.31%, respectively. Forty-five percent of 6B11-iNKT cells were CD4 positive, and there was a weak but significant inverse correlation between total 6B11-iNKT cells and their CD4-positive subset. There was no significant difference in the frequency of iNKT cells between males and females. Conclusions: Results of this study show that the frequency of iNKT cells is fairly high among the Iranian population and suggest matching of study population for age, sex, and ethnic background in iNK-Trelated research. More studies are necessary to understand the factors that can affect the variation in the number of iNKT cells in healthy humans.
AB - Background: Invariant natural killer T cells (iNKT) are a small subset of T lymphocytes which are involved in a wide variety of immune responses. Human studies have reported a wide variation in the number of circulating iNKT cell among healthy donors as well as in some immunologic disorders while there is no or little data about the factors which affect the pool of circulating iNKT cells in human. Given the importance of iNKT cells in immunologic responses and variation in the number and pheno-type of iNKT cells, the aim of this study was to evaluate the frequency of circulating iNKT cells in a cohort of healthy Iranian adults. Methods: The number of circulating iNKT cells was measured among PBMC in a group of healthy Iranian adults by flow cytometry. iNKT cells were identified by using 6B11 monoclonal antibody or by coexpression of TCR Vα24 and Vα11 chain. Results: The mean frequency of 6B11-iNKT cells and Vα24-Vβ11 iNKT cells among T lymphocytes, were 0.54% and 0.31%, respectively. Forty-five percent of 6B11-iNKT cells were CD4 positive, and there was a weak but significant inverse correlation between total 6B11-iNKT cells and their CD4-positive subset. There was no significant difference in the frequency of iNKT cells between males and females. Conclusions: Results of this study show that the frequency of iNKT cells is fairly high among the Iranian population and suggest matching of study population for age, sex, and ethnic background in iNK-Trelated research. More studies are necessary to understand the factors that can affect the variation in the number of iNKT cells in healthy humans.
KW - Flow cytometry
KW - Invariant natural killer T cell
KW - Peripheral blood
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U2 - 10.1002/cyto.b.20489
DO - 10.1002/cyto.b.20489
M3 - Article
C2 - 19714727
AN - SCOPUS:74849087727
SN - 1552-4949
VL - 78
SP - 65
EP - 69
JO - Cytometry Part B - Clinical Cytometry
JF - Cytometry Part B - Clinical Cytometry
IS - 1
ER -