Free malonaldehyde determination in tissues by high-performance liquid chromatography

A. Saari Csallany, Ming Der Guan, John D. Manwaring, Paul B. Addis

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29 Scopus citations


A high-performance liquid chromatographic method was developed for the quantification of free malonaldehyde (MA) in tissues. HPLC separation was performed using a TSK G1000 PW column (7.5-mm i.d. × 30 cm) with a mobile phase of 0.1 m Na3PO4 buffer, pH 8.0, at a flow rate of 0.6 ml/min. The eluant was monitored at 267 nm. Free MA in the tissue sample was separated and quantified in approximately 50 min. The lowest amount of MA that can be determined by this HPLC technique is approximately 1 ng per injection. This method was successfully applied to rat liver and beef, pork, and chicken muscle and was compared to the thiobarbituric acid (TBA) test. It was found to be more sensitive, accurate, and specific for the determination of free MA than the TBA method.

Original languageEnglish (US)
Pages (from-to)277-283
Number of pages7
JournalAnalytical Biochemistry
Issue number2
StatePublished - Nov 1 1984

Bibliographical note

Funding Information:
’ This study was supported by USPHS, NIH Grant 1 ROl ES02325, the Minnesota Agricultural Experiment Station Grant MIN-18-084, and the Minnesota Beef Council. This paper is Scientific Journal Series 13,061, Minnesota Agricultural Experiment Station, St. Paul, Minn. 55108. ’ Abbreviations used: MA, malonaldehyde; TEP, tetraethoxypropane; TBA, thiobarbituric acid, EC, extinction coefficient.


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