Frankia assemblages associated with alnus rubra and alnus viridis are strongly influenced by host species identity

Adam Lipus, Peter G. Kennedy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This study examined the effect of host species identity on the structure of Alnus-associated Frankia bacterial assemblages in the Pacific Northwest, United States, using two approaches. First, Frankia in nodules were sampled from six stands of Alnus rubra or Alnus viridis. Second, a bioassay was conducted where A. rubra and A. viridis seedlings were grown in different soils collected from these two hosts. Frankia genotypes were characterized with nifH sequences and bacterial assemblages were compared using taxon- and divergencebased analyses. Strong host associations were evident in the field; the dominant Frankia genotypes showed significant associations with either A. rubra or A. viridis, and there were host-associated groupings at the assemblage level as well. In the bioassay, host associations among Frankia genotypes were evident but less pronounced, reflecting an interaction between host species and other factors. Although nodule abundance varied among bioassay treatments, seedling dry mass was not strongly correlated with either nodule quantity or soil chemistry. Collectively, our observation and experimental results indicate that host identity is a major factor influencing the genotype composition and abundance of Alnus-associated Frankia assemblages.

Original languageEnglish (US)
Pages (from-to)403-410
Number of pages8
JournalInternational Journal of Plant Sciences
Volume172
Issue number3
DOIs
StatePublished - Mar 1 2011

Fingerprint

Alnus viridis
Alnus rubra
Frankia
genotype
bioassay
Alnus
bioassays
seedling
Northwestern United States
soil chemistry
seedlings

Keywords

  • Alnus
  • Edaphic factors
  • Frankia
  • Host association
  • Microbial ecology
  • NifH
  • Symbiosis

Cite this

@article{e1c5df6e9a4b4979a3dd01fa8e0322a0,
title = "Frankia assemblages associated with alnus rubra and alnus viridis are strongly influenced by host species identity",
abstract = "This study examined the effect of host species identity on the structure of Alnus-associated Frankia bacterial assemblages in the Pacific Northwest, United States, using two approaches. First, Frankia in nodules were sampled from six stands of Alnus rubra or Alnus viridis. Second, a bioassay was conducted where A. rubra and A. viridis seedlings were grown in different soils collected from these two hosts. Frankia genotypes were characterized with nifH sequences and bacterial assemblages were compared using taxon- and divergencebased analyses. Strong host associations were evident in the field; the dominant Frankia genotypes showed significant associations with either A. rubra or A. viridis, and there were host-associated groupings at the assemblage level as well. In the bioassay, host associations among Frankia genotypes were evident but less pronounced, reflecting an interaction between host species and other factors. Although nodule abundance varied among bioassay treatments, seedling dry mass was not strongly correlated with either nodule quantity or soil chemistry. Collectively, our observation and experimental results indicate that host identity is a major factor influencing the genotype composition and abundance of Alnus-associated Frankia assemblages.",
keywords = "Alnus, Edaphic factors, Frankia, Host association, Microbial ecology, NifH, Symbiosis",
author = "Adam Lipus and Kennedy, {Peter G.}",
year = "2011",
month = "3",
day = "1",
doi = "10.1086/658156",
language = "English (US)",
volume = "172",
pages = "403--410",
journal = "International Journal of Plant Sciences",
issn = "1058-5893",
publisher = "University of Chicago",
number = "3",

}

TY - JOUR

T1 - Frankia assemblages associated with alnus rubra and alnus viridis are strongly influenced by host species identity

AU - Lipus, Adam

AU - Kennedy, Peter G.

PY - 2011/3/1

Y1 - 2011/3/1

N2 - This study examined the effect of host species identity on the structure of Alnus-associated Frankia bacterial assemblages in the Pacific Northwest, United States, using two approaches. First, Frankia in nodules were sampled from six stands of Alnus rubra or Alnus viridis. Second, a bioassay was conducted where A. rubra and A. viridis seedlings were grown in different soils collected from these two hosts. Frankia genotypes were characterized with nifH sequences and bacterial assemblages were compared using taxon- and divergencebased analyses. Strong host associations were evident in the field; the dominant Frankia genotypes showed significant associations with either A. rubra or A. viridis, and there were host-associated groupings at the assemblage level as well. In the bioassay, host associations among Frankia genotypes were evident but less pronounced, reflecting an interaction between host species and other factors. Although nodule abundance varied among bioassay treatments, seedling dry mass was not strongly correlated with either nodule quantity or soil chemistry. Collectively, our observation and experimental results indicate that host identity is a major factor influencing the genotype composition and abundance of Alnus-associated Frankia assemblages.

AB - This study examined the effect of host species identity on the structure of Alnus-associated Frankia bacterial assemblages in the Pacific Northwest, United States, using two approaches. First, Frankia in nodules were sampled from six stands of Alnus rubra or Alnus viridis. Second, a bioassay was conducted where A. rubra and A. viridis seedlings were grown in different soils collected from these two hosts. Frankia genotypes were characterized with nifH sequences and bacterial assemblages were compared using taxon- and divergencebased analyses. Strong host associations were evident in the field; the dominant Frankia genotypes showed significant associations with either A. rubra or A. viridis, and there were host-associated groupings at the assemblage level as well. In the bioassay, host associations among Frankia genotypes were evident but less pronounced, reflecting an interaction between host species and other factors. Although nodule abundance varied among bioassay treatments, seedling dry mass was not strongly correlated with either nodule quantity or soil chemistry. Collectively, our observation and experimental results indicate that host identity is a major factor influencing the genotype composition and abundance of Alnus-associated Frankia assemblages.

KW - Alnus

KW - Edaphic factors

KW - Frankia

KW - Host association

KW - Microbial ecology

KW - NifH

KW - Symbiosis

UR - http://www.scopus.com/inward/record.url?scp=79952715802&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952715802&partnerID=8YFLogxK

U2 - 10.1086/658156

DO - 10.1086/658156

M3 - Article

AN - SCOPUS:79952715802

VL - 172

SP - 403

EP - 410

JO - International Journal of Plant Sciences

JF - International Journal of Plant Sciences

SN - 1058-5893

IS - 3

ER -