Abstract
19F NMR spectroscopy of labeled proteins is a sensitive method for characterizing structure, conformational dynamics, higher-order assembly, and ligand binding. Fluorination of aromatic side chains has been suggested as a labeling strategy for small-molecule ligand discovery for protein-protein interaction interfaces. Using a model transcription factor binding domain of the CREB binding protein (CBP)/p300, KIX, we report the first full small-molecule screen using protein-observed 19F NMR spectroscopy. Screening of 508 compounds and validation by 1H-15N HSQC NMR spectroscopy led to the identification of a minimal pharmacaphore for the MLL-KIX interaction site. Hit rate analysis for the CREB-KIX and MLL-KIX sites provided a metric to assess the ligandability or "druggability" of each interface informing future medicinal chemistry efforts. The structural information from the simplified spectra and data collection speed, affords a new screening tool for analysis of protein interfaces and discovery of small molecules. A full small-molecule screen has been achieved using protein-observed 19F NMR (PrOF NMR) spectroscopy of a model transcription factor binding domain of the CREB binding protein (CBP)/p300, KIX. This study demonstrates the applicability of PrOF NMR as a tool for library screening, ligand discovery, and druggability assessment.
Original language | English (US) |
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Pages (from-to) | 3735-3739 |
Number of pages | 5 |
Journal | Angewandte Chemie - International Edition |
Volume | 54 |
Issue number | 12 |
DOIs | |
State | Published - Mar 16 2015 |
Bibliographical note
Publisher Copyright:© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Keywords
- biomolecular NMR
- fluorine
- fragment screening
- protein-protein interactions
- transcription