Fractionation and identification of antioxidant and angiotensin-converting enzyme-inhibitory peptides obtained from plum (Prunus domestica L.) stones

Estefanía González-García, Patrycja Puchalska, María Luisa Marina, María Concepción García

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Peptides with antioxidant and ACE-inhibitory capacities have been obtained from a plum processing by-product. The aim of this work was to fractionate most active plum hydrolysates, to identify peptides in these fractions, and to evaluate their resistance to gastrointestinal enzymes. Highest antioxidant capacity was observed in the whole Alcalase hydrolysate while peptides yielding the highest ability to inhibit ACE were concentrated in the fraction 3kDa from the Thermolysin hydrolysate. RP-HPLC-ESI-MS/MS enabled the identification of 13 and 7 potential antioxidant and ACE inhibitory peptides, respectively, in these fractions. Nevertheless, RP-HPLC alone did not guarantee the successful separation of all peptides. The combination of RP-HPLC and hydrophilic interaction liquid chromatography (HILIC) coupled to ESI-Q-TOF-MS/MS enabled the identification of all peptides. Five potential antioxidant and three potential antihypertensive peptides could be additionally identified by HILIC. A total of 7 potential antioxidant peptides and 3 potential antihypertensive peptides resisted a simulated gastrointestinal digestion.

Original languageEnglish (US)
Pages (from-to)376-384
Number of pages9
JournalJournal of Functional Foods
Volume19
DOIs
StatePublished - Dec 2015
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by the Ministry of Economy and Competitiveness (ref. AGL2012-36362 ), the University of Alcalá (ref. CCG2013/EXP-028 ) and the Comunidad Autónoma de Madrid and European funding from FEDER Program (ref. S2013/ABI-3028 , AVANSECAL). E.G.-G. thanks the University of Alcalá for her pre-doctoral grant. Authors thankfully acknowledge Novozymes Spain S.A. for the kind donation of Alcalase enzyme.

Publisher Copyright:
© 2015 Elsevier Ltd.

Keywords

  • ACE-inhibitory peptides
  • Antioxidant peptides
  • Hydrophilic interaction chromatography
  • Mass spectrometry
  • Plum stone

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