Fourteen novel microsatellite markers for the gopher frog, Lithobates capito (Amphibia: Ranidae)

Schyler O. Nunziata; Stephen C. Richter; Robert D. Denton; John M. Yeiser; Danielle E. Wells; Kenneth L. Jones; Cris Hagen; Stacey L. Lance

doi:10.1007/s12686-011-9508-2

Fourteen novel microsatellite markers for the gopher frog, Lithobates capito (Amphibia: Ranidae)

Schyler O. Nunziata, Stephen C. Richter, Robert D. Denton, John M. Yeiser, Danielle E. Wells, Kenneth L. Jones, Cris Hagen, Stacey L. Lance

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We isolated and characterized a total of 14 microsatellite loci from gopher frogs, Lithobates capito. This species is of conservation concern because most populations have gone locally extinct across the geographic distribution. Loci were screened for 21 individuals from a single population in Florida. The number of alleles per locus ranged from 7 to 17, observed heterozygosity ranged from 0.667 to 0.947, and the probability of identity values ranged from 0.011 to 0.077. These new loci provide tools for examining the genetic diversity and population structure of L. capito populations and addressing factors associated with their decline.

Original language	English (US)
Pages (from-to)	201-203
Number of pages	3
Journal	Conservation Genetics Resources
Volume	4
Issue number	1
DOIs	https://doi.org/10.1007/s12686-011-9508-2
State	Published - Mar 2012
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgments Manuscript preparation was partially supported by the Department of Energy under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation. We thank Daniel Smith for providing the frog specimens.

Keywords

Gopher frog
Lithobates
Microsatellite
PCR primers
Rana
Ranidae
SSR
STR

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10.1007/s12686-011-9508-2

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title = "Fourteen novel microsatellite markers for the gopher frog, Lithobates capito (Amphibia: Ranidae)",

abstract = "We isolated and characterized a total of 14 microsatellite loci from gopher frogs, Lithobates capito. This species is of conservation concern because most populations have gone locally extinct across the geographic distribution. Loci were screened for 21 individuals from a single population in Florida. The number of alleles per locus ranged from 7 to 17, observed heterozygosity ranged from 0.667 to 0.947, and the probability of identity values ranged from 0.011 to 0.077. These new loci provide tools for examining the genetic diversity and population structure of L. capito populations and addressing factors associated with their decline.",

keywords = "Gopher frog, Lithobates, Microsatellite, PCR primers, Rana, Ranidae, SSR, STR",

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note = "Funding Information: Acknowledgments Manuscript preparation was partially supported by the Department of Energy under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation. We thank Daniel Smith for providing the frog specimens.",

year = "2012",

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doi = "10.1007/s12686-011-9508-2",

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AU - Nunziata, Schyler O.

AU - Richter, Stephen C.

AU - Denton, Robert D.

AU - Yeiser, John M.

AU - Wells, Danielle E.

AU - Jones, Kenneth L.

AU - Hagen, Cris

AU - Lance, Stacey L.

N1 - Funding Information: Acknowledgments Manuscript preparation was partially supported by the Department of Energy under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation. We thank Daniel Smith for providing the frog specimens.

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N2 - We isolated and characterized a total of 14 microsatellite loci from gopher frogs, Lithobates capito. This species is of conservation concern because most populations have gone locally extinct across the geographic distribution. Loci were screened for 21 individuals from a single population in Florida. The number of alleles per locus ranged from 7 to 17, observed heterozygosity ranged from 0.667 to 0.947, and the probability of identity values ranged from 0.011 to 0.077. These new loci provide tools for examining the genetic diversity and population structure of L. capito populations and addressing factors associated with their decline.

AB - We isolated and characterized a total of 14 microsatellite loci from gopher frogs, Lithobates capito. This species is of conservation concern because most populations have gone locally extinct across the geographic distribution. Loci were screened for 21 individuals from a single population in Florida. The number of alleles per locus ranged from 7 to 17, observed heterozygosity ranged from 0.667 to 0.947, and the probability of identity values ranged from 0.011 to 0.077. These new loci provide tools for examining the genetic diversity and population structure of L. capito populations and addressing factors associated with their decline.

KW - Gopher frog

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KW - Microsatellite

KW - PCR primers

KW - Rana

KW - Ranidae

KW - SSR

KW - STR

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Fourteen novel microsatellite markers for the gopher frog, Lithobates capito (Amphibia: Ranidae)

Abstract

Bibliographical note

Keywords

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