TY - JOUR
T1 - Formations of Host Fibers and Bacteria in Human Temporal Bones With Otitis Media
AU - Tsuprun, Vladimir
AU - Shibata, Dai
AU - Paparella, Michael
AU - Cureoglu, Sebahattin
N1 - Publisher Copyright:
Copyright © 2021, Otology & Neurotology, Inc.
PY - 2021/8/1
Y1 - 2021/8/1
N2 - Hypothesis:In temporal bones with otitis media, fibrin and neutrophil extracellular traps (NETs) form a fibrous network with bacteria, which is involved in growth of bacterial clusters/biofilms and chronicity of disease.Background:NETs and fibrin are important in host defense against pathogens; however, their role in otitis media is not well understood.Methods:Eight human temporal bones with serous otitis media, 30 with serous-purulent otitis media, 7 with mucoid otitis media, 23 with mucoid-purulent otitis media (OM), 30 with purulent OM, and 30 with chronic otitis media were selected based on histopathologic findings. Fibrous material with bacteria was detected with hematoxylin-eosin, Gram-Weigert, and propidium iodide stains; and its composition was analyzed with immunohistochemistry.Results:Extensive formations of fibrous material with bacteria were observed in 30% of temporal bones with serous-purulent otitis media, 29% with mucoid otitis media, 50% with mucoid-purulent OM, 57% with purulent OM, and 67% of temporal bones with histological evidence of chronic otitis media. Some of these formations showed large bacterial clusters or biofilms. Immunohistochemical analysis showed that fibrous structures were composed of fibrin or NETs.Conclusions:Formations of fibrous material with bacteria were detected in human temporal bones with different types of otitis media. Inflammatory cells were observed mostly in areas with low presence of fibrous structures. The network of fibrous material seems to prevent clearance of bacteria by phagocytic cells and thus influences growth of bacterial clusters or biofilms. Fibrin and NETs may be important for the recurrences and chronicity of disease, and contribute to clogging of tympanostomy tubes in children.
AB - Hypothesis:In temporal bones with otitis media, fibrin and neutrophil extracellular traps (NETs) form a fibrous network with bacteria, which is involved in growth of bacterial clusters/biofilms and chronicity of disease.Background:NETs and fibrin are important in host defense against pathogens; however, their role in otitis media is not well understood.Methods:Eight human temporal bones with serous otitis media, 30 with serous-purulent otitis media, 7 with mucoid otitis media, 23 with mucoid-purulent otitis media (OM), 30 with purulent OM, and 30 with chronic otitis media were selected based on histopathologic findings. Fibrous material with bacteria was detected with hematoxylin-eosin, Gram-Weigert, and propidium iodide stains; and its composition was analyzed with immunohistochemistry.Results:Extensive formations of fibrous material with bacteria were observed in 30% of temporal bones with serous-purulent otitis media, 29% with mucoid otitis media, 50% with mucoid-purulent OM, 57% with purulent OM, and 67% of temporal bones with histological evidence of chronic otitis media. Some of these formations showed large bacterial clusters or biofilms. Immunohistochemical analysis showed that fibrous structures were composed of fibrin or NETs.Conclusions:Formations of fibrous material with bacteria were detected in human temporal bones with different types of otitis media. Inflammatory cells were observed mostly in areas with low presence of fibrous structures. The network of fibrous material seems to prevent clearance of bacteria by phagocytic cells and thus influences growth of bacterial clusters or biofilms. Fibrin and NETs may be important for the recurrences and chronicity of disease, and contribute to clogging of tympanostomy tubes in children.
KW - Bacteria
KW - Bacterial clusters
KW - Biofilms
KW - Fibrin
KW - Fibrous material
KW - Human temporal bones
KW - Middle ear cleft
KW - NETs
KW - Otitis media
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U2 - 10.1097/mao.0000000000003126
DO - 10.1097/mao.0000000000003126
M3 - Article
C2 - 34260514
AN - SCOPUS:85111750898
SN - 1531-7129
VL - 42
SP - E949-E957
JO - Otology and Neurotology
JF - Otology and Neurotology
IS - 7
ER -