Formation of mRNA 3' termini: stability and dissociation of a complex involving the AAUAAA sequence.

D. Zarkower, M. Wickens

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Formation of the 3' termini of mRNAs in animal cells involves endonucleolytic cleavage of a pre-mRNA, followed by polyadenylation of the newly formed end. Here we demonstrate that, during cleavage in vitro, the highly conserved AAUAAA sequence of the pre-mRNA forms a complex with a factor present in a crude nuclear extract. This complex is required for cleavage and polyadenylation. It normally is transient, but is very stable on cleaved RNA to which a single terminal cordycepin residue has been added. The complex can form either during the cleavage reaction, or on a synthetic RNA that ends at the polyadenylation site. Mutations which prevent cleavage also prevent complex formation. The complex dissociates during or after polyadenylation, enabling the released activities to catalyze a second round of cleavage.

Original languageEnglish (US)
Pages (from-to)177-186
Number of pages10
JournalEMBO Journal
Volume6
Issue number1
StatePublished - Jan 1 1987

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