Formation of gap junctions by treatment in vitro with potassium conductance blockers

M. S. Kannan, E. E. Daniel

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Gap junctions were regularly seen in thin sections of canine tracheal smooth muscle incubated in vitro. Their number was increased in tissues exposed in vitro to either two potassium conductance blockers, tetraethylammonium (TEA) and 4-aminopyridine (4-AP), and at the same time the muscles became mechanically active, with spontaneous contractions. The presence of gap junctions in this smooth muscle may provide one basis for cell-to-cell coupling, and their increase after TEA- and 4-AP-treatment could account for a decreased junctional resistance between cells, contributing to a longer space constant. However, an increase in gap junctions was not sufficient to change the bahavior of trachealis smooth muscle from multiunit to single-unit type. Gap junctions in increased numbers persisted after washout of 4-AP, which caused inhibition of spontaneous contractions, and despite inhibition of the contractile effects of 4-AP by atropine. The rapid induction of gap junction formation was not dependent on de novo synthesis of protein. The fact that the number of gap junctions can be increased by chemical agents has important implications for control of their formation and provides a tool for analysis of their role in cell-to-cell coupling.

Original languageEnglish (US)
Pages (from-to)338-348
Number of pages11
JournalJournal of Cell Biology
Volume78
Issue number2
DOIs
StatePublished - 1978

Bibliographical note

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

Fingerprint Dive into the research topics of 'Formation of gap junctions by treatment in vitro with potassium conductance blockers'. Together they form a unique fingerprint.

Cite this