Formation of a DNA layer on Langmuir-Blodgett films and its enzymatic digestion

Anusarka Bhaumik, Madhugiri Ramakanth, Loveleen Kaur Brar, Arup K. Raychaudhuri, Francis Rondelez, Dipankar Chatterji

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Here, we report a system we have developed where long double-stranded DNAs (dsDNAs) are immobilized on a monolayer of Zn-arachidate. We have applied the Langmuir-Blodgett technique to form the monolayer of Zn-arachidate where Zn(II) is bound to arachidic acid through charge neutralization. Because tetrahedral Zn(II) participates in DNA recognition through coordination, we have been able to layer DNA over the Zn-arachidate monolayer. The DNA layer shows a typical compression and expansion cycle in a concentration-dependent fashion. Interestingly, the DNA monolayer is available for enzymatic degradation by DNasel. The detection of DNA and its accessibility towards biological reaction is demonstrated by imaging through fluorescence microscopy. The conformation of the DNA, immobilized on the monolayer, was studied with the help of atomic force microscopy (AFM). We observed that the dsDNAs were aligned in a stretched manner on the surface. To investigate further, we also demonstrate here that the small single-stranded DNA (ssDNA) immobilized on the air-water interface can act as a target molecule for the complementary ssDNA present in the subphase. The study of DNA hybridization done with the help of fluorescence spectroscopy clearly supports the AFM characterization.

Original languageEnglish (US)
Pages (from-to)5891-5896
Number of pages6
JournalLangmuir
Volume20
Issue number14
DOIs
StatePublished - Jul 6 2004

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