Formation and analysis of heterocyclic aromatic amine-DNA adducts in vitro and in vivo

Robert J. Turesky, Paul Vouros

Research output: Contribution to journalReview article

86 Scopus citations

Abstract

The detection and quantification of heterocyclic aromatic amine (HAA)-DNA adducts, critical biomarkers in interspecies extrapolation of toxicity data for human risk assessment, remains a challenging analytical problem. The two main analytical methods currently in use to screen for HAA-DNA adducts are the 32P-postlabeling assay and mass spectrometry, using either accelerated mass spectrometry (AMS) or liquid chromatography and electrospray ionization mass spectrometry (LC-ESI-MS). In this review, the principal methods to synthesize and characterize DNA adducts, and the methods applied to measure HAA-DNA adduct in vitro and vivo are discussed.

Original languageEnglish (US)
Pages (from-to)155-166
Number of pages12
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume802
Issue number1
DOIs
StatePublished - Mar 25 2004

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Keywords

  • DNA
  • Heterocyclic aromatic amine-DNA adducts
  • Reviews

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