Force and function: probing proteins with AFM-based force spectroscopy

Elias M. Puchner, Hermann E. Gaub

Research output: Contribution to journalReview articlepeer-review

196 Scopus citations

Abstract

Forces play a pivotal role in life, and the response of live systems to forces requires molecules and molecular interactions with adequate properties to counteract both in a passive and also, if needed, in an active, dynamic manner. However, at the level of individual molecules these forces are so minute, that the development of sophisticated experiments to measure and control them was required. With the maturation of these techniques, particularly the AFM-based single-molecule force spectroscopy into commercial instruments, the scope has widened considerably and more and more studies shed light onto the different aspects of biomolecular mechanics. Many surprises turned up and more are waiting for us.

Original languageEnglish (US)
Pages (from-to)605-614
Number of pages10
JournalCurrent Opinion in Structural Biology
Volume19
Issue number5
DOIs
StatePublished - Oct 2009

Bibliographical note

Funding Information:
We thank Ann Fornof and Stefan Stahl for helpful discussions. This work was funded by the Deutsche Forschungsgemeinschaft and the Nanosystems Initiative Munich (NIM).

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