Focusing capillary optics for use in solution small-angle X-ray scattering

Jessica S. Lamb; Sterling Cornaby; Kurt Andresen; Lisa Kwok; Hye Yoon Park; Xiangyun Qiu; Detlef M. Smilgies; Donald H. Bilderback; Lois Pollack

doi:10.1107/S0021889806044505

Focusing capillary optics for use in solution small-angle X-ray scattering

Jessica S. Lamb, Sterling Cornaby, Kurt Andresen, Lisa Kwok, Hye Yoon Park, Xiangyun Qiu, Detlef M. Smilgies, Donald H. Bilderback, Lois Pollack

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Measurements of the global conformation of macromolecules can be carried out using small-angle X-ray scattering (SAXS). Glass focusing capillaries, manufactured at the Cornell High Energy Synchrotron Source (CHESS), have been successfully employed for SAXS measurements on the heme protein cytochrome c. These capillaries provide high X-ray flux into a spot size of tens of micrometres, permitting short exposures of small-volume samples. Such a capability is ideal for use in conjunction with microfluidic mixers, where time resolution may be determined by beam size and sample volumes are kept small to facilitate mixing and conserve material.

Original language	English (US)
Article number	ce5006
Pages (from-to)	193-195
Number of pages	3
Journal	Journal of Applied Crystallography
Volume	40
Issue number	1
DOIs	https://doi.org/10.1107/S0021889806044505
State	Published - Jan 12 2007
Externally published	Yes

Keywords

Focusing capillary optics
Macromolecular crystallography

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10.1107/S0021889806044505

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abstract = "Measurements of the global conformation of macromolecules can be carried out using small-angle X-ray scattering (SAXS). Glass focusing capillaries, manufactured at the Cornell High Energy Synchrotron Source (CHESS), have been successfully employed for SAXS measurements on the heme protein cytochrome c. These capillaries provide high X-ray flux into a spot size of tens of micrometres, permitting short exposures of small-volume samples. Such a capability is ideal for use in conjunction with microfluidic mixers, where time resolution may be determined by beam size and sample volumes are kept small to facilitate mixing and conserve material.",

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T1 - Focusing capillary optics for use in solution small-angle X-ray scattering

AU - Lamb, Jessica S.

AU - Cornaby, Sterling

AU - Andresen, Kurt

AU - Kwok, Lisa

AU - Park, Hye Yoon

AU - Qiu, Xiangyun

AU - Smilgies, Detlef M.

AU - Bilderback, Donald H.

AU - Pollack, Lois

PY - 2007/1/12

Y1 - 2007/1/12

N2 - Measurements of the global conformation of macromolecules can be carried out using small-angle X-ray scattering (SAXS). Glass focusing capillaries, manufactured at the Cornell High Energy Synchrotron Source (CHESS), have been successfully employed for SAXS measurements on the heme protein cytochrome c. These capillaries provide high X-ray flux into a spot size of tens of micrometres, permitting short exposures of small-volume samples. Such a capability is ideal for use in conjunction with microfluidic mixers, where time resolution may be determined by beam size and sample volumes are kept small to facilitate mixing and conserve material.

AB - Measurements of the global conformation of macromolecules can be carried out using small-angle X-ray scattering (SAXS). Glass focusing capillaries, manufactured at the Cornell High Energy Synchrotron Source (CHESS), have been successfully employed for SAXS measurements on the heme protein cytochrome c. These capillaries provide high X-ray flux into a spot size of tens of micrometres, permitting short exposures of small-volume samples. Such a capability is ideal for use in conjunction with microfluidic mixers, where time resolution may be determined by beam size and sample volumes are kept small to facilitate mixing and conserve material.

KW - Focusing capillary optics

KW - Macromolecular crystallography

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Focusing capillary optics for use in solution small-angle X-ray scattering

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