Focal middle cerebral artery ischemia in rats via a transfemoral approach using a custom designed microwire

Afshin A. Divani; Ricky Chow; Homayoun Sadeghi-Bazargani; Amanda J. Murphy; Jessica A. Nordberg; Julian V. Tokarev; Mario Hevesi; Xiao Wang; Xiao Hong Zhu; Tommy Acompanado; Peter A. Edwards; Yi Zhang; Wei Chen

doi:10.1136/neurintsurg-2014-011607

Focal middle cerebral artery ischemia in rats via a transfemoral approach using a custom designed microwire

Afshin A. Divani, Ricky Chow, Homayoun Sadeghi-Bazargani, Amanda J. Murphy, Jessica A. Nordberg, Julian V. Tokarev, Mario Hevesi, Xiao Wang, Xiao Hong Zhu, Tommy Acompanado, Peter A. Edwards, Yi Zhang, Wei Chen

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Objectives The aim of this study was to develop a reliable and repeatable method of inducing focal middle cerebral artery occlusion (MCAo) in rats without ligation of the external carotid artery (ECA), while reducing the risk of subarachnoid hemorrhage. Methods We prototyped microwires with different diameters (0.0120 inch, 0.0115 inch, 0.0110 inch), materials, and construction methods (coil-on-core, extruded polymer jacket-on-core). Under fluoroscopic guidance and using femoral artery access, the microwires were navigated into the internal carotid artery of male Wistar rats (n=50, weight 376±64 g) to induce MCAo for 1 or 2 h. We performed neurological assessments at baseline, and at 3, 24, 72, and 168 h after MCAo. MRI measurements were performed on a 9.4 T scanner at 1 and 7 days post-injury. Results The 0.0115 inch microwire with polymer jacket-on-core provided the most successful outcome. At 1 and 7 days post-injury, we observed similar infarction volumes for 1 and 2 h MCAo in the MRI study. Infarcted lesion volumes in both MCAo groups were significantly reduced at 7 days compared with 1 day post-injury. The trend in longitudinal changes for the scores of different neurological assessments was confirmed to be significant after the injury, but both groups showed a similar trend of neurological deficits over the course of the study. Conclusions We have developed a reliable and repeatable MCAo method in rats, allowing for precise occlusion of the MCA under direct fluoroscopic visualization without alteration of the cerebral hemodynamics associated with ECA ligation. The custom designed microwire can also be sized for targeted focal ischemia in larger animals.

Original language	English (US)
Pages (from-to)	608-614
Number of pages	7
Journal	Journal of NeuroInterventional Surgery
Volume	8
Issue number	6
DOIs	https://doi.org/10.1136/neurintsurg-2014-011607
State	Published - Jun 2016

Bibliographical note

Funding Information:
This work was supported in part by the Summer Student Fellowship program at the Stroke Research Center, University of Minnesota, that was established following generous support from HealthEZ (Bloomington, Minnesota, USA), Lake Region Medical (Chaska, Minnesota, USA), and Covidien (Mansfield, Massachusetts, USA). JVT has received a Seed Grant from the American Medical Association and the Carolyn L Cuckein Student Research Grant from AOA Honor Medical Society. AJM has received a Medical Student Scholarship in Cerebrovascular Disease and Stroke sponsored by the American Heart Association's Stroke Council. MH was also supported by a Nelson Heart Scholarship from the American Heart Association. AAD has received research support from Lake Region Medical

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Publisher link

10.1136/neurintsurg-2014-011607

Find It

Find It at U of M Libraries

Cite this

Divani, A. A., Chow, R., Sadeghi-Bazargani, H., Murphy, A. J., Nordberg, J. A., Tokarev, J. V., Hevesi, M., Wang, X., Zhu, X. H., Acompanado, T., Edwards, P. A., Zhang, Y., & Chen, W. (2016). Focal middle cerebral artery ischemia in rats via a transfemoral approach using a custom designed microwire. Journal of NeuroInterventional Surgery, 8(6), 608-614. https://doi.org/10.1136/neurintsurg-2014-011607

Divani, AA, Chow, R, Sadeghi-Bazargani, H, Murphy, AJ, Nordberg, JA, Tokarev, JV, Hevesi, M, Wang, X, Zhu, XH, Acompanado, T, Edwards, PA, Zhang, Y & Chen, W 2016, 'Focal middle cerebral artery ischemia in rats via a transfemoral approach using a custom designed microwire', Journal of NeuroInterventional Surgery, vol. 8, no. 6, pp. 608-614. https://doi.org/10.1136/neurintsurg-2014-011607

@article{f29aa8235adb48f9ba7f99aec2d4f28c,

title = "Focal middle cerebral artery ischemia in rats via a transfemoral approach using a custom designed microwire",

abstract = "Objectives The aim of this study was to develop a reliable and repeatable method of inducing focal middle cerebral artery occlusion (MCAo) in rats without ligation of the external carotid artery (ECA), while reducing the risk of subarachnoid hemorrhage. Methods We prototyped microwires with different diameters (0.0120 inch, 0.0115 inch, 0.0110 inch), materials, and construction methods (coil-on-core, extruded polymer jacket-on-core). Under fluoroscopic guidance and using femoral artery access, the microwires were navigated into the internal carotid artery of male Wistar rats (n=50, weight 376±64 g) to induce MCAo for 1 or 2 h. We performed neurological assessments at baseline, and at 3, 24, 72, and 168 h after MCAo. MRI measurements were performed on a 9.4 T scanner at 1 and 7 days post-injury. Results The 0.0115 inch microwire with polymer jacket-on-core provided the most successful outcome. At 1 and 7 days post-injury, we observed similar infarction volumes for 1 and 2 h MCAo in the MRI study. Infarcted lesion volumes in both MCAo groups were significantly reduced at 7 days compared with 1 day post-injury. The trend in longitudinal changes for the scores of different neurological assessments was confirmed to be significant after the injury, but both groups showed a similar trend of neurological deficits over the course of the study. Conclusions We have developed a reliable and repeatable MCAo method in rats, allowing for precise occlusion of the MCA under direct fluoroscopic visualization without alteration of the cerebral hemodynamics associated with ECA ligation. The custom designed microwire can also be sized for targeted focal ischemia in larger animals.",

author = "Divani, {Afshin A.} and Ricky Chow and Homayoun Sadeghi-Bazargani and Murphy, {Amanda J.} and Nordberg, {Jessica A.} and Tokarev, {Julian V.} and Mario Hevesi and Xiao Wang and Zhu, {Xiao Hong} and Tommy Acompanado and Edwards, {Peter A.} and Yi Zhang and Wei Chen",

note = "Funding Information: This work was supported in part by the Summer Student Fellowship program at the Stroke Research Center, University of Minnesota, that was established following generous support from HealthEZ (Bloomington, Minnesota, USA), Lake Region Medical (Chaska, Minnesota, USA), and Covidien (Mansfield, Massachusetts, USA). JVT has received a Seed Grant from the American Medical Association and the Carolyn L Cuckein Student Research Grant from AOA Honor Medical Society. AJM has received a Medical Student Scholarship in Cerebrovascular Disease and Stroke sponsored by the American Heart Association's Stroke Council. MH was also supported by a Nelson Heart Scholarship from the American Heart Association. AAD has received research support from Lake Region Medical",

year = "2016",

month = jun,

doi = "10.1136/neurintsurg-2014-011607",

language = "English (US)",

volume = "8",

pages = "608--614",

journal = "Journal of NeuroInterventional Surgery",

issn = "1759-8478",

publisher = "BMJ Publishing Group",

number = "6",

}

TY - JOUR

T1 - Focal middle cerebral artery ischemia in rats via a transfemoral approach using a custom designed microwire

AU - Divani, Afshin A.

AU - Chow, Ricky

AU - Sadeghi-Bazargani, Homayoun

AU - Murphy, Amanda J.

AU - Nordberg, Jessica A.

AU - Tokarev, Julian V.

AU - Hevesi, Mario

AU - Wang, Xiao

AU - Zhu, Xiao Hong

AU - Acompanado, Tommy

AU - Edwards, Peter A.

AU - Zhang, Yi

AU - Chen, Wei

N1 - Funding Information: This work was supported in part by the Summer Student Fellowship program at the Stroke Research Center, University of Minnesota, that was established following generous support from HealthEZ (Bloomington, Minnesota, USA), Lake Region Medical (Chaska, Minnesota, USA), and Covidien (Mansfield, Massachusetts, USA). JVT has received a Seed Grant from the American Medical Association and the Carolyn L Cuckein Student Research Grant from AOA Honor Medical Society. AJM has received a Medical Student Scholarship in Cerebrovascular Disease and Stroke sponsored by the American Heart Association's Stroke Council. MH was also supported by a Nelson Heart Scholarship from the American Heart Association. AAD has received research support from Lake Region Medical

PY - 2016/6

Y1 - 2016/6

N2 - Objectives The aim of this study was to develop a reliable and repeatable method of inducing focal middle cerebral artery occlusion (MCAo) in rats without ligation of the external carotid artery (ECA), while reducing the risk of subarachnoid hemorrhage. Methods We prototyped microwires with different diameters (0.0120 inch, 0.0115 inch, 0.0110 inch), materials, and construction methods (coil-on-core, extruded polymer jacket-on-core). Under fluoroscopic guidance and using femoral artery access, the microwires were navigated into the internal carotid artery of male Wistar rats (n=50, weight 376±64 g) to induce MCAo for 1 or 2 h. We performed neurological assessments at baseline, and at 3, 24, 72, and 168 h after MCAo. MRI measurements were performed on a 9.4 T scanner at 1 and 7 days post-injury. Results The 0.0115 inch microwire with polymer jacket-on-core provided the most successful outcome. At 1 and 7 days post-injury, we observed similar infarction volumes for 1 and 2 h MCAo in the MRI study. Infarcted lesion volumes in both MCAo groups were significantly reduced at 7 days compared with 1 day post-injury. The trend in longitudinal changes for the scores of different neurological assessments was confirmed to be significant after the injury, but both groups showed a similar trend of neurological deficits over the course of the study. Conclusions We have developed a reliable and repeatable MCAo method in rats, allowing for precise occlusion of the MCA under direct fluoroscopic visualization without alteration of the cerebral hemodynamics associated with ECA ligation. The custom designed microwire can also be sized for targeted focal ischemia in larger animals.

AB - Objectives The aim of this study was to develop a reliable and repeatable method of inducing focal middle cerebral artery occlusion (MCAo) in rats without ligation of the external carotid artery (ECA), while reducing the risk of subarachnoid hemorrhage. Methods We prototyped microwires with different diameters (0.0120 inch, 0.0115 inch, 0.0110 inch), materials, and construction methods (coil-on-core, extruded polymer jacket-on-core). Under fluoroscopic guidance and using femoral artery access, the microwires were navigated into the internal carotid artery of male Wistar rats (n=50, weight 376±64 g) to induce MCAo for 1 or 2 h. We performed neurological assessments at baseline, and at 3, 24, 72, and 168 h after MCAo. MRI measurements were performed on a 9.4 T scanner at 1 and 7 days post-injury. Results The 0.0115 inch microwire with polymer jacket-on-core provided the most successful outcome. At 1 and 7 days post-injury, we observed similar infarction volumes for 1 and 2 h MCAo in the MRI study. Infarcted lesion volumes in both MCAo groups were significantly reduced at 7 days compared with 1 day post-injury. The trend in longitudinal changes for the scores of different neurological assessments was confirmed to be significant after the injury, but both groups showed a similar trend of neurological deficits over the course of the study. Conclusions We have developed a reliable and repeatable MCAo method in rats, allowing for precise occlusion of the MCA under direct fluoroscopic visualization without alteration of the cerebral hemodynamics associated with ECA ligation. The custom designed microwire can also be sized for targeted focal ischemia in larger animals.

UR - http://www.scopus.com/inward/record.url?scp=84969277757&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84969277757&partnerID=8YFLogxK

U2 - 10.1136/neurintsurg-2014-011607

DO - 10.1136/neurintsurg-2014-011607

M3 - Article

C2 - 25935923

AN - SCOPUS:84969277757

SN - 1759-8478

VL - 8

SP - 608

EP - 614

JO - Journal of NeuroInterventional Surgery

JF - Journal of NeuroInterventional Surgery

IS - 6

ER -

Focal middle cerebral artery ischemia in rats via a transfemoral approach using a custom designed microwire

Abstract

Bibliographical note

UN SDGs

Publisher link

Find It

Other files and links

Fingerprint

Cite this