Abstract
Objectives The aim of this study was to develop a reliable and repeatable method of inducing focal middle cerebral artery occlusion (MCAo) in rats without ligation of the external carotid artery (ECA), while reducing the risk of subarachnoid hemorrhage. Methods We prototyped microwires with different diameters (0.0120 inch, 0.0115 inch, 0.0110 inch), materials, and construction methods (coil-on-core, extruded polymer jacket-on-core). Under fluoroscopic guidance and using femoral artery access, the microwires were navigated into the internal carotid artery of male Wistar rats (n=50, weight 376±64 g) to induce MCAo for 1 or 2 h. We performed neurological assessments at baseline, and at 3, 24, 72, and 168 h after MCAo. MRI measurements were performed on a 9.4 T scanner at 1 and 7 days post-injury. Results The 0.0115 inch microwire with polymer jacket-on-core provided the most successful outcome. At 1 and 7 days post-injury, we observed similar infarction volumes for 1 and 2 h MCAo in the MRI study. Infarcted lesion volumes in both MCAo groups were significantly reduced at 7 days compared with 1 day post-injury. The trend in longitudinal changes for the scores of different neurological assessments was confirmed to be significant after the injury, but both groups showed a similar trend of neurological deficits over the course of the study. Conclusions We have developed a reliable and repeatable MCAo method in rats, allowing for precise occlusion of the MCA under direct fluoroscopic visualization without alteration of the cerebral hemodynamics associated with ECA ligation. The custom designed microwire can also be sized for targeted focal ischemia in larger animals.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 608-614 |
| Number of pages | 7 |
| Journal | Journal of NeuroInterventional Surgery |
| Volume | 8 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 2016 |
Fingerprint
Cite this
Focal middle cerebral artery ischemia in rats via a transfemoral approach using a custom designed microwire. / Divani, Afshin A.; Chow, Ricky; Sadeghi-Bazargani, Homayoun; Murphy, Amanda J.; Nordberg, Jessica A.; Tokarev, Julian V.; Hevesi, Mario; Wang, Xiao; Zhu, Xiao Hong; Acompanado, Tommy; Edwards, Peter A.; Zhang, Yi; Chen, Wei.
In: Journal of NeuroInterventional Surgery, Vol. 8, No. 6, 06.2016, p. 608-614.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Focal middle cerebral artery ischemia in rats via a transfemoral approach using a custom designed microwire
AU - Divani, Afshin A.
AU - Chow, Ricky
AU - Sadeghi-Bazargani, Homayoun
AU - Murphy, Amanda J.
AU - Nordberg, Jessica A.
AU - Tokarev, Julian V.
AU - Hevesi, Mario
AU - Wang, Xiao
AU - Zhu, Xiao Hong
AU - Acompanado, Tommy
AU - Edwards, Peter A.
AU - Zhang, Yi
AU - Chen, Wei
PY - 2016/6
Y1 - 2016/6
N2 - Objectives The aim of this study was to develop a reliable and repeatable method of inducing focal middle cerebral artery occlusion (MCAo) in rats without ligation of the external carotid artery (ECA), while reducing the risk of subarachnoid hemorrhage. Methods We prototyped microwires with different diameters (0.0120 inch, 0.0115 inch, 0.0110 inch), materials, and construction methods (coil-on-core, extruded polymer jacket-on-core). Under fluoroscopic guidance and using femoral artery access, the microwires were navigated into the internal carotid artery of male Wistar rats (n=50, weight 376±64 g) to induce MCAo for 1 or 2 h. We performed neurological assessments at baseline, and at 3, 24, 72, and 168 h after MCAo. MRI measurements were performed on a 9.4 T scanner at 1 and 7 days post-injury. Results The 0.0115 inch microwire with polymer jacket-on-core provided the most successful outcome. At 1 and 7 days post-injury, we observed similar infarction volumes for 1 and 2 h MCAo in the MRI study. Infarcted lesion volumes in both MCAo groups were significantly reduced at 7 days compared with 1 day post-injury. The trend in longitudinal changes for the scores of different neurological assessments was confirmed to be significant after the injury, but both groups showed a similar trend of neurological deficits over the course of the study. Conclusions We have developed a reliable and repeatable MCAo method in rats, allowing for precise occlusion of the MCA under direct fluoroscopic visualization without alteration of the cerebral hemodynamics associated with ECA ligation. The custom designed microwire can also be sized for targeted focal ischemia in larger animals.
AB - Objectives The aim of this study was to develop a reliable and repeatable method of inducing focal middle cerebral artery occlusion (MCAo) in rats without ligation of the external carotid artery (ECA), while reducing the risk of subarachnoid hemorrhage. Methods We prototyped microwires with different diameters (0.0120 inch, 0.0115 inch, 0.0110 inch), materials, and construction methods (coil-on-core, extruded polymer jacket-on-core). Under fluoroscopic guidance and using femoral artery access, the microwires were navigated into the internal carotid artery of male Wistar rats (n=50, weight 376±64 g) to induce MCAo for 1 or 2 h. We performed neurological assessments at baseline, and at 3, 24, 72, and 168 h after MCAo. MRI measurements were performed on a 9.4 T scanner at 1 and 7 days post-injury. Results The 0.0115 inch microwire with polymer jacket-on-core provided the most successful outcome. At 1 and 7 days post-injury, we observed similar infarction volumes for 1 and 2 h MCAo in the MRI study. Infarcted lesion volumes in both MCAo groups were significantly reduced at 7 days compared with 1 day post-injury. The trend in longitudinal changes for the scores of different neurological assessments was confirmed to be significant after the injury, but both groups showed a similar trend of neurological deficits over the course of the study. Conclusions We have developed a reliable and repeatable MCAo method in rats, allowing for precise occlusion of the MCA under direct fluoroscopic visualization without alteration of the cerebral hemodynamics associated with ECA ligation. The custom designed microwire can also be sized for targeted focal ischemia in larger animals.
UR - http://www.scopus.com/inward/record.url?scp=84969277757&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84969277757&partnerID=8YFLogxK
U2 - 10.1136/neurintsurg-2014-011607
DO - 10.1136/neurintsurg-2014-011607
M3 - Article
C2 - 25935923
AN - SCOPUS:84969277757
VL - 8
SP - 608
EP - 614
JO - Journal of NeuroInterventional Surgery
JF - Journal of NeuroInterventional Surgery
SN - 1759-8478
IS - 6
ER -