Fluoroquinolone-resistant extraintestinal Escherichia coli clinical isolates representing the O15: K52: H1 clonal group from humans and dogs in Australia

Joanne L. Platell; Rowland N. Cobbold; James R. Johnson; Connie R. Clabots; Darren J. Trott

doi:10.1016/j.cimid.2012.02.002

Fluoroquinolone-resistant extraintestinal Escherichia coli clinical isolates representing the O15: K52: H1 clonal group from humans and dogs in Australia

Joanne L. Platell, Rowland N. Cobbold, James R. Johnson, Connie R. Clabots, Darren J. Trott

Medicine - Veteran's Administration Medical Center

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7 Scopus citations

Abstract

Antimicrobial-resistant extraintestinal pathogenic Escherichia coli (ExPEC) impact both human and veterinary medicine. One ExPEC clonal group that has become increasingly multidrug-resistant is serotype O15:K52:H1. Accordingly, we sought O15:K52:H1 strains among fluoroquinolone-resistant (FQ^r) E. coli clinical isolates from humans (n=582) and dogs (n=120) in Australia. The phylogenetic group D isolates (267/702; 38%) were screened for O15:K52:H1-specific single-nucleotide polymorphisms (SNPs) in fumC and the O15 rfb variant. The 34 so-identified O15:K52:H1 isolates (33 human, 1 canine) underwent antimicrobial susceptibility profiling, virulence genotyping, and macrorestriction profiling. Although susceptibility profiles varied, the 34 isolates were closely related by pulsed-field gel electrophoresis and exhibited typical O15:K52:H1-associated virulence profiles (complete pap operon, F16 papA allele, papG allele II, iha, fimH, sat, fyuA, iutA, kpsMII, ompT). The canine isolate closely resembled human isolates. Thus, O15:K52:H1 strains contribute to the FQ^r ExPEC population in Australia and may potentially be transferred between humans and dogs.

Original language	English (US)
Pages (from-to)	319-324
Number of pages	6
Journal	Comparative Immunology, Microbiology and Infectious Diseases
Volume	35
Issue number	4
DOIs	https://doi.org/10.1016/j.cimid.2012.02.002
State	Published - Jul 2012

Bibliographical note

Funding Information:
This work was supported by an Australian Research Council Linkage project with Bayer Health Care AG and The University of Queensland (D.J.T. and R.N.C.) and by the Office of Research and Development, Medical Research Service, Department of Veterans Affairs (J.R.J.). The study sponsors imposed no commercial influence in study design, interpretation of data, or submission of the manuscript.

Keywords

Clonal group O15:K52:H1
Dogs
Extraintestinal pathogenic Escherichia coli
Fluoroquinolone resistance

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10.1016/j.cimid.2012.02.002

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Fluoroquinolone-resistant extraintestinal Escherichia coli clinical isolates representing the O15: K52: H1 clonal group from humans and dogs in Australia. / Platell, Joanne L.; Cobbold, Rowland N.; Johnson, James R. et al.
In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 35, No. 4, 07.2012, p. 319-324.

Research output: Contribution to journal › Article › peer-review

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title = "Fluoroquinolone-resistant extraintestinal Escherichia coli clinical isolates representing the O15: K52: H1 clonal group from humans and dogs in Australia",

abstract = "Antimicrobial-resistant extraintestinal pathogenic Escherichia coli (ExPEC) impact both human and veterinary medicine. One ExPEC clonal group that has become increasingly multidrug-resistant is serotype O15:K52:H1. Accordingly, we sought O15:K52:H1 strains among fluoroquinolone-resistant (FQr) E. coli clinical isolates from humans (n=582) and dogs (n=120) in Australia. The phylogenetic group D isolates (267/702; 38\%) were screened for O15:K52:H1-specific single-nucleotide polymorphisms (SNPs) in fumC and the O15 rfb variant. The 34 so-identified O15:K52:H1 isolates (33 human, 1 canine) underwent antimicrobial susceptibility profiling, virulence genotyping, and macrorestriction profiling. Although susceptibility profiles varied, the 34 isolates were closely related by pulsed-field gel electrophoresis and exhibited typical O15:K52:H1-associated virulence profiles (complete pap operon, F16 papA allele, papG allele II, iha, fimH, sat, fyuA, iutA, kpsMII, ompT). The canine isolate closely resembled human isolates. Thus, O15:K52:H1 strains contribute to the FQr ExPEC population in Australia and may potentially be transferred between humans and dogs.",

keywords = "Clonal group O15:K52:H1, Dogs, Extraintestinal pathogenic Escherichia coli, Fluoroquinolone resistance",

author = "Platell, \{Joanne L.\} and Cobbold, \{Rowland N.\} and Johnson, \{James R.\} and Clabots, \{Connie R.\} and Trott, \{Darren J.\}",

note = "Funding Information: This work was supported by an Australian Research Council Linkage project with Bayer Health Care AG and The University of Queensland (D.J.T. and R.N.C.) and by the Office of Research and Development, Medical Research Service, Department of Veterans Affairs (J.R.J.). The study sponsors imposed no commercial influence in study design, interpretation of data, or submission of the manuscript. ",

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AU - Johnson, James R.

AU - Clabots, Connie R.

AU - Trott, Darren J.

N1 - Funding Information: This work was supported by an Australian Research Council Linkage project with Bayer Health Care AG and The University of Queensland (D.J.T. and R.N.C.) and by the Office of Research and Development, Medical Research Service, Department of Veterans Affairs (J.R.J.). The study sponsors imposed no commercial influence in study design, interpretation of data, or submission of the manuscript.

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N2 - Antimicrobial-resistant extraintestinal pathogenic Escherichia coli (ExPEC) impact both human and veterinary medicine. One ExPEC clonal group that has become increasingly multidrug-resistant is serotype O15:K52:H1. Accordingly, we sought O15:K52:H1 strains among fluoroquinolone-resistant (FQr) E. coli clinical isolates from humans (n=582) and dogs (n=120) in Australia. The phylogenetic group D isolates (267/702; 38%) were screened for O15:K52:H1-specific single-nucleotide polymorphisms (SNPs) in fumC and the O15 rfb variant. The 34 so-identified O15:K52:H1 isolates (33 human, 1 canine) underwent antimicrobial susceptibility profiling, virulence genotyping, and macrorestriction profiling. Although susceptibility profiles varied, the 34 isolates were closely related by pulsed-field gel electrophoresis and exhibited typical O15:K52:H1-associated virulence profiles (complete pap operon, F16 papA allele, papG allele II, iha, fimH, sat, fyuA, iutA, kpsMII, ompT). The canine isolate closely resembled human isolates. Thus, O15:K52:H1 strains contribute to the FQr ExPEC population in Australia and may potentially be transferred between humans and dogs.

AB - Antimicrobial-resistant extraintestinal pathogenic Escherichia coli (ExPEC) impact both human and veterinary medicine. One ExPEC clonal group that has become increasingly multidrug-resistant is serotype O15:K52:H1. Accordingly, we sought O15:K52:H1 strains among fluoroquinolone-resistant (FQr) E. coli clinical isolates from humans (n=582) and dogs (n=120) in Australia. The phylogenetic group D isolates (267/702; 38%) were screened for O15:K52:H1-specific single-nucleotide polymorphisms (SNPs) in fumC and the O15 rfb variant. The 34 so-identified O15:K52:H1 isolates (33 human, 1 canine) underwent antimicrobial susceptibility profiling, virulence genotyping, and macrorestriction profiling. Although susceptibility profiles varied, the 34 isolates were closely related by pulsed-field gel electrophoresis and exhibited typical O15:K52:H1-associated virulence profiles (complete pap operon, F16 papA allele, papG allele II, iha, fimH, sat, fyuA, iutA, kpsMII, ompT). The canine isolate closely resembled human isolates. Thus, O15:K52:H1 strains contribute to the FQr ExPEC population in Australia and may potentially be transferred between humans and dogs.

KW - Clonal group O15:K52:H1

KW - Dogs

KW - Extraintestinal pathogenic Escherichia coli

KW - Fluoroquinolone resistance

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JO - Comparative Immunology, Microbiology and Infectious Diseases

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ER -

Fluoroquinolone-resistant extraintestinal Escherichia coli clinical isolates representing the O15: K52: H1 clonal group from humans and dogs in Australia

Abstract

Bibliographical note

Keywords

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