Fluorogold administration via microdialysis labels neurons terminating within the dialysis region

Stephen R. Skilling, Mary A. Mullett, Al J Beitz, Alice A Larson

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The use of microdialysis to monitor the release of neurotransmitters in selected regions of the CNS has increased substantially in the last several years. We describe here a method for retrogradely labeling neurons that terminate within the effective sampling region surrounding the dialysis cannula. This is accomplished by direct infusion of fluorogold through the dialysis cannula. By combining this technique with immunohistochemistry, it is possible to identify neurons that could contribute to the neurotransmitter release measured by microdialysis.

Original languageEnglish (US)
Pages (from-to)85-90
Number of pages6
JournalJournal of Neuroscience Methods
Volume41
Issue number1
DOIs
StatePublished - Jan 1992

Keywords

  • Fluorogold
  • Immunohistochemistry
  • Microdialysis
  • Neurotransmitter
  • Raphe magnus
  • Spinal cord
  • Vestibular nucleus

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