The use of microdialysis to monitor the release of neurotransmitters in selected regions of the CNS has increased substantially in the last several years. We describe here a method for retrogradely labeling neurons that terminate within the effective sampling region surrounding the dialysis cannula. This is accomplished by direct infusion of fluorogold through the dialysis cannula. By combining this technique with immunohistochemistry, it is possible to identify neurons that could contribute to the neurotransmitter release measured by microdialysis.
Bibliographical noteFunding Information:
This research was supported by US Public Health Service Grants DA04090, DA04190, and DA00124 to A.A.L, CA01342 to S.R.S., and DA06687, DE06682 and NS19208 to A.J.B. The use and care of animals in this study was performed in accordance with the guidelines of the Minnesota Animal Care and Use Committee and those prepared by the Committee on Care and Use of Laboratory Animals of the Institute of Laboratory Animal Resources, Nation Research Council (DHEW Publication no. (N1H) 78-23, revised 1978).
- Raphe magnus
- Spinal cord
- Vestibular nucleus