Fluorogold administration via microdialysis labels neurons terminating within the dialysis region

Stephen R. Skilling; Mary A. Mullett; Al J Beitz; Alice A Larson

doi:10.1016/0165-0270(92)90126-X

Fluorogold administration via microdialysis labels neurons terminating within the dialysis region

Stephen R. Skilling, Mary A. Mullett, Al J Beitz, Alice A Larson

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The use of microdialysis to monitor the release of neurotransmitters in selected regions of the CNS has increased substantially in the last several years. We describe here a method for retrogradely labeling neurons that terminate within the effective sampling region surrounding the dialysis cannula. This is accomplished by direct infusion of fluorogold through the dialysis cannula. By combining this technique with immunohistochemistry, it is possible to identify neurons that could contribute to the neurotransmitter release measured by microdialysis.

Original language	English (US)
Pages (from-to)	85-90
Number of pages	6
Journal	Journal of Neuroscience Methods
Volume	41
Issue number	1
DOIs	https://doi.org/10.1016/0165-0270(92)90126-X
State	Published - Jan 1992

Keywords

Fluorogold
Immunohistochemistry
Microdialysis
Neurotransmitter
Raphe magnus
Spinal cord
Vestibular nucleus

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title = "Fluorogold administration via microdialysis labels neurons terminating within the dialysis region",

abstract = "The use of microdialysis to monitor the release of neurotransmitters in selected regions of the CNS has increased substantially in the last several years. We describe here a method for retrogradely labeling neurons that terminate within the effective sampling region surrounding the dialysis cannula. This is accomplished by direct infusion of fluorogold through the dialysis cannula. By combining this technique with immunohistochemistry, it is possible to identify neurons that could contribute to the neurotransmitter release measured by microdialysis.",

keywords = "Fluorogold, Immunohistochemistry, Microdialysis, Neurotransmitter, Raphe magnus, Spinal cord, Vestibular nucleus",

author = "Skilling, {Stephen R.} and Mullett, {Mary A.} and Beitz, {Al J} and Larson, {Alice A}",

year = "1992",

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AU - Skilling, Stephen R.

AU - Mullett, Mary A.

AU - Beitz, Al J

AU - Larson, Alice A

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AB - The use of microdialysis to monitor the release of neurotransmitters in selected regions of the CNS has increased substantially in the last several years. We describe here a method for retrogradely labeling neurons that terminate within the effective sampling region surrounding the dialysis cannula. This is accomplished by direct infusion of fluorogold through the dialysis cannula. By combining this technique with immunohistochemistry, it is possible to identify neurons that could contribute to the neurotransmitter release measured by microdialysis.

KW - Fluorogold

KW - Immunohistochemistry

KW - Microdialysis

KW - Neurotransmitter

KW - Raphe magnus

KW - Spinal cord

KW - Vestibular nucleus

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