Fluorescent Pyrene Derivative of Concanavalin A: Preparation and Spectroscopic Characterization

Brian A. Herman, Salvador M. Fernandez

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The preparation and characterization of a fluorescent 1-pyreneacrylate-concanavalin A conjugate are described. Excitation, emission, and polarization spectra are presented as well as quantum yield and fluorescence lifetimes. Perrin plots examining the rotational behavior of the pyrene-Con A complex show good agreement with those previously published for other fluorophore-Con A conjugates. The suitability of this flurorphore as a donor for resonance energy transfer studies is discussed.

Original languageEnglish (US)
Pages (from-to)3271-3275
Number of pages5
JournalBiochemistry
Volume21
Issue number14
DOIs
StatePublished - Jul 1982

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