Fluorescent, prussian blue-based biocompatible nanoparticle system for multimodal imaging contrast

László Forgách, Nikolett Hegedűs, Ildikó Horváth, Bálint Kiss, Noémi Kovács, Zoltán Varga, Géza Jakab, Tibor Kovács, Parasuraman Padmanabhan, Krisztián Szigeti, Domokos Máthé

Research output: Contribution to journalArticlepeer-review

Abstract

(1) Background. The main goal of this work was to develop a fluorescent dye-labelling technique for our previously described nanosized platform, citrate-coated Prussian blue (PB) nanoparticles (PBNPs). In addition, characteristics and stability of the PB nanoparticles labelled with fluorescent dyes were determined. (2) Methods. We adsorbed the fluorescent dyes Eosin Y and Rhodamine B and methylene blue (MB) to PB-nanoparticle systems. The physicochemical properties of these fluorescent dye-labeled PBNPs (iron(II);iron(III);octadecacyanide) were determined using atomic force microscopy, dynamic light scattering, zeta potential measurements, scanning-and transmission electron microscopy, X-ray diffraction, and Fourier-transformation infrared spectroscopy. A methylene-blue (MB) labelled, polyethylene-glycol stabilized PBNP platform was selected for further assessment of in vivo distribution and fluorescent imaging after intravenous administration in mice. (3) Results. The MB-labelled particles emitted a strong fluorescent signal at 662 nm. We found that the fluorescent light emission and steric stabilization made this PBNP-MB particle platform applicable for in vivo optical imaging. (4) Conclusion. We successfully produced a fluorescent and stable, Prussian blue-based nanosystem. The particles can be used as a platform for imaging contrast enhancement. In vivo stability and biodistribution studies revealed new aspects of the use of PBNPs.

Original languageEnglish (US)
Article number1732
Pages (from-to)1-15
Number of pages15
JournalNanomaterials
Volume10
Issue number9
DOIs
StatePublished - Sep 2020
Externally publishedYes

Bibliographical note

Funding Information:
Funding: The research leading to these results has received funding from the European Union’s Seventh Framework Programme (FP7/2007–2013) under Grant Agreements HEALTH-F2-2011-278850 (INMiND), FP7 HEALTH-305311 (INSERT), and TÁMOP-4.2.1./B-09/1/KMR-2010–0001. K.SZ. was supported by the Janos Bolyai Research Fellowship Program of the Hungarian Academy of Sciences. T.J.K. acknowledges support from the Hungarian Government`s Operative Programme of Economics and Innovation Support, grant number GINOP 2.3.2-15-2016-00016. Part of the research was financed by the Thematic Excellence Programme (TKP) of the Ministry of Innovation and Technology of Hungary, within the framework of the BIOImaging Excellence programme at Semmelweis University. COST Action CA16122 supported part of our work. Part of this study was supported by the National Research, Development and Innovation Office of Hungary (NKFIA; NVKP-16-1-2016-0017 National Heart Program).

Funding Information:
Acknowledgments: The authors acknowledge the helpful discussions with Miklós Kellermayer (Department of Biophysics and Radiation Biology, Semmelweis University). The research leading to these results has received funding from the European Union’s Seventh Framework Programme (FP7/2007–2013) under Grant Agreements HEALTH-F2-2011-278850 (INMiND), FP7 HEALTH-305311 (INSERT), and TÁMOP-4.2.1./B-09/1/KMR-2010– 0001. K.S. was supported by the Janos Bolyai Research Fellowship Program of the Hungarian Academy of Sciences. T.J.K. acknowledges support from the Hungarian Government`s Operative Programme of Economics and Innovation Support, grant number GINOP 2.3.2-15-2016-00016. Part of the research was financed by the Thematic Excellence Programme (TKP) of the Ministry of Innovation and Technology of Hungary, within the framework of the BIOImaging Excellence programme at Semmelweis University. COST Action CA16122 supported part of our work. Part of this study was supported by the National Research, Development and Innovation Office of Hungary (NKFIA; NVKP-16-1-2016-0017 National Heart Program).

Keywords

  • Biocompatible
  • Fluorescent imaging
  • Optical imaging
  • Prussian blue nanoparticles

PubMed: MeSH publication types

  • Journal Article

Fingerprint Dive into the research topics of 'Fluorescent, prussian blue-based biocompatible nanoparticle system for multimodal imaging contrast'. Together they form a unique fingerprint.

Cite this