Abstract
Better living through biochemistry: Phosphorylation of a Cy5-labeled Abl kinase peptide biosensor resulted in an extension of fluorescence lifetime in live cells. Time-correlated single photon counting fluorescence lifetime imaging (FLIM) enabled excellent signal-to-noise to visualize the subcellular patterns of this sensor (see picture). This strategy should be generalizable to other peptide-based kinase substrates for imaging of kinase activity in single cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3931-3934 |
| Number of pages | 4 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 52 |
| Issue number | 14 |
| DOIs | |
| State | Published - Apr 2 2013 |
Keywords
- Abl kinase
- biosensors
- fluorescence lifetime imaging microscopy
- fluorescent probes
- inhibitors
