Fluorescence lifetime imaging of biosensor peptide phosphorylation in single live cells

Nur P. Damayanti, Laurie L. Parker, Joseph M K Irudayaraj

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Better living through biochemistry: Phosphorylation of a Cy5-labeled Abl kinase peptide biosensor resulted in an extension of fluorescence lifetime in live cells. Time-correlated single photon counting fluorescence lifetime imaging (FLIM) enabled excellent signal-to-noise to visualize the subcellular patterns of this sensor (see picture). This strategy should be generalizable to other peptide-based kinase substrates for imaging of kinase activity in single cells.

Original languageEnglish (US)
Pages (from-to)3931-3934
Number of pages4
JournalAngewandte Chemie - International Edition
Volume52
Issue number14
DOIs
StatePublished - Apr 2 2013

Keywords

  • Abl kinase
  • biosensors
  • fluorescence lifetime imaging microscopy
  • fluorescent probes
  • inhibitors

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