Fluorescence Lifetime Imaging Microscopy (FLIM): Instrumentation and Applications

Xue Feng Wang, Ammasi Periasamy, Brian Herman, David M. Coleman

Research output: Contribution to journalArticlepeer-review

110 Scopus citations


The new and novel techniques of fluorescence lifetime imaging (FLI), and fluorescence lifetime imaging microscopy (FLIM) provide the investigator with the capacity to quantitate two-dimensional fluorescence intensity distributions and lifetimes. The concept, theory, and instrumentation of FLI and FLIM are reviewed in this paper. The implementation of FLIM instrumentation with conventional and confocal microscopic systems is discussed. These instruments permit the quantitative measurement of molecular interactions and chemical environment from samples in biological, physical, and environmental sciences. Numerous applications in the biomedical sciences for FLIM instrumentation are also discussed.

Original languageEnglish (US)
Pages (from-to)369-395
Number of pages27
JournalCritical Reviews in Analytical Chemistry
Issue number5
StatePublished - Jan 1992


  • confocal fluorescence microscopy
  • fluorescence lifetime imaging
  • fluorescence lifetime imaging microscopy
  • fluorescence microscopy
  • fluorescence resonance energy transfer
  • fluorescence spectroscopy
  • image intensifier
  • instrumentation
  • time-resolved fluorescence microscopy
  • time-resolved fluorescence spectroscopy


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