Fluorescence in situ hybridization analysis reveals multiple loci of knob-associated DNA elements in one-knob and knobless maize lines

Sami S M Adawy, Robert M. Stupar, Jiming Jiang

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Fluorescence in situ hybridization analyses were conducted to examine the presence or absence of the 180- and 350-bp knob-associated tandem repeats in maize strains previously defined as "one-knob" or "knobless. " Multiple loci were found to hybridize to these two repeats in all maize lines analyzed. Our results show that the number of 180- and 350-bp repeat loci do not correlate with the number of knobs in maize and that these tandem repeats are not independently sufficient to confer knob heterochromatin, even when present at megabase sizes.

Original languageEnglish (US)
Pages (from-to)1113-1116
Number of pages4
JournalJournal of Histochemistry and Cytochemistry
Volume52
Issue number8
DOIs
StatePublished - Aug 1 2004

Keywords

  • Fiber-fluorescence in situ hybridization
  • Fluorescence in situ
  • Heterochromatin
  • Hybridization
  • Knob
  • Tandem repeat

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